The current study investigates and compares the biological effects of ultrathin conformal coatings of zirconium dioxide (ZrO2) and vanadium pentoxide (V2O5) on osteoblastic MG-63 cells grown on TiO2 nanotube layers (TNTs). Coatings were achieved by the atomic layer deposition (ALD) technique. TNTs with average tube diameters of 15, 30, and 100 nm were fabricated on Ti substrates (via electrochemical anodization) and were used as primary substrates for the study. The MG-63 cell growth and proliferation after 48 h of incubation on hybrid TNTs/ZrO2 and TNTs/V2O5 surfaces was evaluated in comparison to the uncoated TNTs of each diameter. The density of viable MG-63 cells was assessed for all the TNT surfaces, along with the cell morphology and the spreading behavior (i.e., the cell length). The ultrathin coatings retained the original morphology of the TNTs but changed the surface chemical composition, wettability, and cell behavior, whose interplay is the subject of the present investigation. These findings offer interesting views on the influence of the composition of biomedical implant surfaces, triggered by ALD ultrathin coatings on them. The outcomes of this work shed light on the assessment of the biocompatibility of the two different ALD coatings.

• Klíčová slova
• MG-63, TiO2 nanotube layers, ZrO2, V2O5, atomic layer deposition, cell viability.,
• MeSH
• biokompatibilní potahované materiály * chemie   farmakologie   MeSH
• buněčné linie MeSH
• elektrody MeSH
• lidé MeSH
• nanotrubičky * chemie   MeSH
• osteoblasty * účinky léků   cytologie   MeSH
• povrchové vlastnosti MeSH
• proliferace buněk * účinky léků   MeSH
• sloučeniny vanadu chemie   farmakologie   MeSH
• titan * chemie   farmakologie   MeSH
• zirkonium * chemie   farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• biokompatibilní potahované materiály * MeSH
• sloučeniny vanadu MeSH
• titan * MeSH
• titanium dioxide MeSH Prohlížeč
• vanadium pentoxide MeSH Prohlížeč
• zirconium oxide MeSH Prohlížeč
• zirkonium * MeSH

An in-depth investigation was conducted on a promising composite material (BiVO4/TiO2), focusing on its potential toxicity, photoinduced catalytic properties, as well as its antibiofilm and antimicrobial functionalities. The preparation process involved the synthesis of 2D TiO2 using the lyophilization method, which was subsequently functionalized with sphere-like BiVO4 through wet impregnation. Finally, we developed BiVO4/TiO2 S-scheme heterojunctions which can greatly promote the separation of electron-hole pairs to achieve high photocatalytic performance. The evaluation of concentration- and time-dependent viability inhibition was performed on human lung carcinoma epithelial A549 cells. This assessment included the estimation of glutathione levels and mitochondrial dehydrogenase activity. Significantly, the BiVO4/TiO2 composite demonstrated minimal toxicity towards A549 cells. Impressively, the BiVO4/TiO2 composite exhibited notable photocatalytic performance in the degradation of rhodamine B (k=0.135 min-1) and phenol (k=0.016 min-1). In terms of photoinduced antimicrobial performance, the composite effectively inactivated both gram-negative E. coli and gram-positive E. faecalis bacteria upon 60 minutes of UV-A light exposure, resulting in a significant log 6 (log 10 CFU/mL) reduction in bacterial count. In addition, a 49 % reduction of E. faecalis biofilm was observed. These promising results can be attributed to the unique 2D morphology of TiO2 modified by sphere-like BiVO4, leading to an increased generation of (intracellular) hydroxyl radicals, which plays a crucial role in the treatments of both organic pollutants and bacteria. This research has significant potential for various applications, particularly in addressing environmental contamination and microbial infections.

• Klíčová slova
• 2D TiO2, antibiofilm, antimicrobial, cytotoxicity, photocatalysis, sphere-like BiVO4,
• MeSH
• antibakteriální látky * chemie   farmakologie   MeSH
• biofilmy účinky léků   MeSH
• bismut * chemie   MeSH
• buňky A549 MeSH
• Enterococcus faecalis účinky léků   MeSH
• Escherichia coli * účinky léků   MeSH
• fotochemické procesy * MeSH
• fotolýza MeSH
• katalýza MeSH
• lidé MeSH
• nanostruktury chemie   MeSH
• rhodaminy chemie   MeSH
• titan * chemie   MeSH
• vanadáty * chemie   farmakologie   MeSH
• viabilita buněk účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky * MeSH
• bismut * MeSH
• bismuth vanadium tetraoxide MeSH Prohlížeč
• rhodamine B MeSH Prohlížeč
• rhodaminy MeSH
• titan * MeSH
• titanium dioxide MeSH Prohlížeč
• vanadáty * MeSH

A series of five decavanadates (V10) using a simple, one-pot synthesis, adhering to the model template: transition metal ion - decavanadate - ligands:(Hnicotinamide)2{[Co(H2O)3(nicotinamide)2]2[μ-V10O28]}.6H2O (1), {[Co(H2O)4(isonicotinamide)2]3}V10O28·4H2O (2), {[Co(H2O)4]2[Co(H2O)2(μ-pyrazinamide)2][μ-V10O28]}·4H2O (3) {[Co(H2O)4(μ-pyrazinamide)]3.V10O28}·4H2O (4), and (NH4)2{[Ni(H2O)4(2-hydroxyethylpyridine)]2}V10O28·2H2O (5) was synthesized. X-ray analysis reveals that 1 and 3 are decavanadato complexes, while 2, 4 and 5 are decavanadate complex salts. Moreover, 3 is the first example of a polymeric decavanadato complex, employing direct coordination with the metal center and the organic ligand, in toto. From the solution studies using 51V NMR spectroscopy, it was decoded that 1 and 3 stay stable in the model buffer solution and aqueous media. Binding to model proteins, cytotoxicity and water oxidation catalysis (WOC) was studied primarily for 1 and 3 and concluded that neither 1 nor 3 have an interaction with the model proteins thaumatin, lysozyme and proteinase K, because of the presence of the organic ligands in the Co(II) center, any further interplay with the proteins was blocked. Cytotoxicity studies reveal that 1 is 40% less toxic (0.05 mM) and 26% less toxic (0.1 mM) than the uncoordinated V10 with human cell lines A549 and HeLa respectively. In WOC, 1 performed superior activity, by evolving 143.37 nmol of O2 which is 700% (9-fold) increase than the uncoordinated V10.

• Klíčová slova
• Bioinorganic chemistry, Cell viability, Cytotoxicity, Decavanadate, Protein binding, Water oxidation catalysis,
• MeSH
• anionty MeSH
• katalýza MeSH
• kobalt * chemie   MeSH
• lidé MeSH
• ligandy MeSH
• pyrazinamid MeSH
• vanadáty * chemie   MeSH
• voda chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• anionty MeSH
• kobalt * MeSH
• ligandy MeSH
• pyrazinamid MeSH
• vanadáty * MeSH
• voda MeSH

To investigate the effect of vanadyl trehalose (VT) on oxidative stress and reduced glutathione/glutathione-S-transferase (GSH/GSTs) pathway gene expression in mouse gastrointestinal tract, as well as the protective effects of vitamin C (VC) and reduced glutathione (GSH). Thirty male Kunming mice were randomly divided into five groups: control group (group A), VT group (group B), VC + VT group (group C), GSH + VT group (group D) and VC + GSH + VT group (group E). The content of reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) activity and the expressions of glutamate-cysteine ligase catalytic subunit (GCLC), glutathione synthetase (GSS), regulated through glutathione reductase (GSR) and glutathione-S-transferase pi (GSTpi) in stomach and duodenum in vanadyl trehalose treated group were lower than those in group A (P<0.05). The C, D, E group can significantly improve the above indicators, but those only in the stomach in E group reached the level of the control group. Vanadyl trehalose (VT) was able to cause oxidative stress damage to the gastrointestinal tract of mice, which affects GSH content and GSH-Px activity and interferes with the normal expression of GSH/GSTs pathway. Exogenous vitamin C, reduced glutathione and the combination of the two could play a specific role in antioxidant protection and reduce the toxicity of vanadyl trehalose.

• MeSH
• antioxidancia farmakologie   MeSH
• biomimetika MeSH
• glutathion metabolismus   MeSH
• glutathionperoxidasa metabolismus   MeSH
• glutathionreduktasa metabolismus   MeSH
• glutathiontransferasa metabolismus   MeSH
• hypoglykemika farmakologie   MeSH
• inzulin farmakologie   MeSH
• kyselina askorbová farmakologie   MeSH
• myši MeSH
• oxidační stres MeSH
• trehalosa farmakologie   MeSH
• vanadáty farmakologie   MeSH
• žaludek účinky léků   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antioxidancia MeSH
• glutathion MeSH
• glutathionperoxidasa MeSH
• glutathionreduktasa MeSH
• glutathiontransferasa MeSH
• hypoglykemika MeSH
• inzulin MeSH
• kyselina askorbová MeSH
• trehalosa MeSH
• vanadáty MeSH

Yeasts play a key role in the production of alcoholic beverages by fermentation processes. However, because of their continuous growth, they commonly cause spoilage of the final product. Herein, we introduce dual magnetic/light-responsive self-propelled microrobots that can actively move in a beer sample and capture yeast cells. The presence of magnetic nanoparticles on the surface of the microrobots enables their magnetic actuation under fuel-free conditions. In addition, their photoactivity under visible-light irradiation leads to an overall enhancement of their swimming and yeast removal capabilities. It was found that after the application of the microrobots into a real unfiltered beer sample, these micromachines were able to remove almost 100 % of residual yeasts. In addition, these microrobots could also be added at the initial step of the fermentation process without altering the final beer properties, such as alcohol level, color, and pH. This work demonstrates the potential of using externally actuated microrobots as an innovative and low-cost solution for avoiding yeast spoilage in complex liquid environments, such as alcoholic beverages. Therefore, these autonomous self-propelled microrobots open new avenues for future applications in the food industry.

• Klíčová slova
• beer spoilage, fermentation, micromotors, photocatalysis, yeast removal,
• MeSH
• antibakteriální látky chemie   MeSH
• bismut chemie   MeSH
• fermentace * MeSH
• fotochemické procesy MeSH
• kontaminace potravin prevence a kontrola   MeSH
• magnetické nanočástice chemie   MeSH
• magnetické pole MeSH
• mikrosféry MeSH
• peroxid vodíku chemie   MeSH
• pivo mikrobiologie   MeSH
• Saccharomyces cerevisiae metabolismus   MeSH
• světlo MeSH
• vanadáty chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• bismut MeSH
• bismuth vanadium tetraoxide MeSH Prohlížeč
• magnetické nanočástice MeSH
• peroxid vodíku MeSH
• vanadáty MeSH

AIM: To evaluate the effects of orally administered gadolinium orthovanadate GdVO4:Eu3+ nanoparticles (VNPs) on the course of chronic carrageenan-induced intestinal inflammation. METHODS: Samples of small intestinal tissue were collected from four groups of rats (intact, after administration of VNPs, with carrageenaninduced intestinal inflammation, with carrageenan-induced intestinal inflammation orally exposed to VNPs) to assess the intestinal morphology and HSP90α expression. Levels of seromucoid, C-reactive protein, TNF-α, IL-1β and IL-10 were determined in blood serum. RESULTS: Oral exposure to VNPs was associated with neither elevation of inflammation markers in blood serum nor HSP90α overexpression in the small intestine, i.e. no toxic effects of VNPs were observed. Carrageenan-induced intestinal inflammation was accompanied by higher levels of TNF-α and IL-1β, as well as HSP90α upregulation in the intestinal mucosa, compared with controls. Administration of VNPs to rats with enteritis did not lead to statistically significant changes in concentrations of circulating pro-inflammatory cytokines with the trend towards their increase. CONCLUSION: No adverse effects were observed in rats orally exposed to VNPs at a dose of 20 μg/kg during two weeks. Using the experimental model of carrageenan-induced enteritis, it was demonstrated that VNPs at the dose used in our study did not affect the course of intestinal inflammation.

• Klíčová slova
• HSP90α, carrageenan, intestinal inflammation, nanoparticles, rats,
• MeSH
• C-reaktivní protein účinky léků   metabolismus   MeSH
• enterokolitida krev   chemicky indukované   patologie   MeSH
• gadolinium farmakologie   MeSH
• idiopatické střevní záněty krev   patologie   MeSH
• interleukin-10 krev   MeSH
• interleukin-1beta krev   účinky léků   MeSH
• karagenan toxicita   MeSH
• kovové nanočástice * MeSH
• krysa rodu Rattus MeSH
• modely nemocí na zvířatech MeSH
• orosomukoid účinky léků   metabolismus   MeSH
• proteiny tepelného šoku HSP90 účinky léků   metabolismus   MeSH
• scavengery volných radikálů farmakologie   MeSH
• střevní sliznice účinky léků   metabolismus   patologie   MeSH
• TNF-alfa krev   účinky léků   MeSH
• vanadáty farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• C-reaktivní protein MeSH
• gadolinium MeSH
• Hsp90aa1 protein, rat MeSH Prohlížeč
• IL1B protein, rat MeSH Prohlížeč
• interleukin-10 MeSH
• interleukin-1beta MeSH
• karagenan MeSH
• orosomukoid MeSH
• proteiny tepelného šoku HSP90 MeSH
• scavengery volných radikálů MeSH
• TNF-alfa MeSH
• vanadáty MeSH

The increasing risk of acute large-scale exposure of ionising irradiation on the population underlines the necessity of developing effective radioprotective and mitigating agents. The aim of this work was to investigate the effect of sodium orthovanadate pre-treatment on mice exposed to high doses of gamma rays (from 5 to 13 Gy). The determination of median lethal dose within 30 days confirmed that orthovanadate applied to total-body-irradiated mice intra-peritoneally has a radioprotective but not a mitigating effect. With orthovanadate pre-treatment, the composition of cellularity in the bone marrow improved substantially and the main lymphocyte populations restored during the first month after irradiation. These findings contribute to 'gap-filling' in radioprotective effects and demonstrate the importance of haematological parameters in radiation-response prediction.

• MeSH
• B-lymfocyty účinky léků   MeSH
• buňky NK účinky léků   MeSH
• celotělové ozáření * MeSH
• inhibitor p21 cyklin-dependentní kinasy metabolismus   MeSH
• ionizující záření MeSH
• kostní dřeň účinky léků   účinky záření   MeSH
• lymfocyty účinky léků   účinky záření   MeSH
• makrofágy metabolismus   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• nádorový supresorový protein p53 metabolismus   MeSH
• průtoková cytometrie MeSH
• radioprotektivní látky farmakologie   MeSH
• T-lymfocyty účinky léků   MeSH
• vanadáty farmakologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• Cdkn1a protein, mouse MeSH Prohlížeč
• inhibitor p21 cyklin-dependentní kinasy MeSH
• nádorový supresorový protein p53 MeSH
• radioprotektivní látky MeSH
• Trp53 protein, mouse MeSH Prohlížeč
• vanadáty MeSH

Light-driven micro/nanomotors represent the next generation of automotive devices that can be easily actuated and controlled by using an external light source. As the field evolves, there is a need for developing more sophisticated micromachines that can fulfill diverse tasks in complex environments. Herein, we introduce single-component BiVO4 micromotors with well-defined micro/nanostructures that can swim both individually and as collectively assembled entities under visible-light irradiation. These devices can perform cargo loading and transport of passive particles as well as living microorganisms without any surface functionalization. Interestingly, after photoactivation, the BiVO4 micromotors exhibited an ability to seek and adhere to yeast cell walls, with the possibility to control their attachment/release by switching the light on/off, respectively. Taking advantage of the selective motor/fungal cells attachment, the fungicidal activity of BiVO4 micromotors under visible illumination was also demonstrated. The presented star-shaped BiVO4 micromotors, obtained by a hydrothermal synthesis, contribute to the potential large-scale fabrication of light-powered micromotors. Moreover, these multifunctional single-component micromachines with controlled self-propulsion, collective behavior, cargo transportation, and photocatalytic activity capabilities hold promising applications in sensing, biohybrids assembly, cargo delivery, and microbiological water pollution remediation.

• Klíčová slova
• bismuth vanadate, cargo transport, fungicidal activity, micromotors, visible light,
• MeSH
• bismut chemie   MeSH
• elektrochemické techniky MeSH
• nanostruktury chemie   MeSH
• povrchové vlastnosti MeSH
• Saccharomyces cerevisiae cytologie   izolace a purifikace   MeSH
• světlo * MeSH
• vanadáty chemická syntéza   chemie   MeSH
• velikost částic MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bismut MeSH
• bismuth vanadium tetraoxide MeSH Prohlížeč
• vanadáty MeSH

The family Fabaceae traditionally serves as a food and herbal remedies source. Certain plants serve for treatment of menopausal symptoms based on a presence of typical secondary metabolites, isoflavones. Beside soybean and clovers, other plants or cultures in vitro can produce these molecules. A cultivation in vitro can be enhanced by elicitation that stimulates metabolites biosynthesis via stress reaction. Vanadium compounds have been already described as potential elicitors, and the aim of this study was to determine the impact of NH₄VO₃ and VOSO₄ solutions on isoflavones production in Genista tinctoria L. cell cultures. The significant increase of isoflavones content, such as genistin, genistein, or formononetin, was measured in a nutrient medium or dry mass after NH₄VO₃ treatment for 24 or 48 h. The possible transport mechanism of isoflavones release as a result of elicitation was further evaluated. An incubation with different transport inhibitors prior to elicitation took effect on isoflavones content in the medium. However, there was a non-ended result for particular metabolites such as genistein and daidzein, where ATP-binding cassette (ABC) or, alternatively, multidrug and toxin extrusion (MATE) proteins can participate. Possible elicitation by some inhibitors was discussed as a result of their pleiotropic effect. Despite this outcome, the determination of the transport mechanism is an important step for identification of the specific transporter.

• Klíčová slova
• Dyer’s Greenweed, elicitation, heavy metals, plasma membrane transport,
• MeSH
• buněčné kultury metody   MeSH
• Genista chemie   cytologie   účinky léků   MeSH
• isoflavony chemie   MeSH
• sekundární metabolismus účinky léků   MeSH
• sloučeniny vanadu farmakologie   MeSH
• vanadáty farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• ammonium metavanadate MeSH Prohlížeč
• isoflavony MeSH
• sloučeniny vanadu MeSH
• vanadáty MeSH
• vanadyl sulfate MeSH Prohlížeč

Hyperglycemia is the most important contributor in the onset and progress of diabetic complications mainly by producing oxidative stress. The present study was carried out to observe, the antihyperglycemic effect of sodium orthovanadate (SOV) and Trigonella foenum graecum seed powder (TSP) administration on blood glucose and insulin levels, membrane linked enzymes (monoamine oxidase, acetylcholinesterase, Ca2+ATPase), intracellular calcium (Ca2+) levels, lipid peroxidation, membrane fluidity and neurolipofuscin accumulation in brain of the alloxan induced diabetic rats and to see whether the treatment with SOV and TSP was capable of reversing the diabetic effects. Diabetes was induced by administration of alloxan monohydrate (15 mg/100 g body weight) and rats were treated with 2 IU insulin, 0.6 mg/ml SOV, 5% TSP in the diet and a combination of 0.2 mg/ml SOV and 5% TSP separately for three weeks. Diabetic rats showed hyperglycemia with almost four fold high blood glucose levels. Activities of acetylcholinesterase and Ca2+ATPase decreased in diabetic rat brain. Diabetic rats exhibited an increased level of intracellular Ca2+ levels, lipid peroxidation, neurolipofuscin accumulations and monoamine oxidase activity. Treatment of diabetic rats with insulin, TSP, SOV and a combined therapy of lower dose of SOV with TSP revived normoglycemia and restored the altered level of membrane bound enzymes, lipid peroxidation and neurolipofuscin accumulation. Our results showed that lower doses of SOV (0.2 mg/ml) could be used in combination with TSP in normalization of altered metabolic parameters and membrane linked enzymes without any harmful side effect.

• MeSH
• experimentální diabetes mellitus farmakoterapie   MeSH
• inzulin krev   MeSH
• krevní glukóza analýza   MeSH
• krysa rodu Rattus MeSH
• lipofuscin metabolismus   MeSH
• monoaminoxidasa metabolismus   MeSH
• mozek - chemie MeSH
• oxidační stres MeSH
• peroxidace lipidů MeSH
• potkani Wistar MeSH
• rostlinné extrakty aplikace a dávkování   farmakologie   MeSH
• semena rostlinná chemie   MeSH
• Trigonella chemie   MeSH
• vanadáty aplikace a dávkování   farmakologie   MeSH
• vápník analýza   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• inzulin MeSH
• krevní glukóza MeSH
• lipofuscin MeSH
• monoaminoxidasa MeSH
• rostlinné extrakty MeSH
• vanadáty MeSH
• vápník MeSH