BACKGROUND: Telomere length is a biomarker of cellular aging, influenced by various environmental and lifestyle factors. Air pollution is a known environmental stressor that may impact telomere dynamics. This study aimed to investigate the effect of age, lifetime exposure to air pollution, inflammatory parameters and selected lifestyle factors on telomere length. METHODS: The study included 356 participants aged 35-65 living in two regions with varying pollution. Telomere length was measured using qPCR. Individual lifetime exposures to PM10, PM2.5, NO2, benzo(a)pyrene and benzene were calculated based on historical air quality data. Statistical analysis of age, pollution exposure, inflammatory parameters, and lifestyle factors on telomere length was performed using logistic regression and generalized linear models, with odds ratios calculated. RESULTS: Unexpectedly, higher air pollutants lifetime exposures were associated with longer telomeres, particularly for PM10 51-55 μg/m3 (OR = 5.67, p < 0.001), PM2.5 42-45 μg/m3 (OR = 6.56, p < 0.001), B(a)P 6.9-8.3 ng/m3 (OR = 5.25, p = 0.002), NO2 26-27 μg/m3 (OR = 5.22, p = 0.001) and benzene 2.45-2.75 μg/m3 (OR = 6.13, p < 0.001). Age significantly affected telomere length, with older individuals having shorter telomeres. Socioeconomic factors such as college education were positively associated with longer telomeres, while lifestyle factors did not show significant associations. IL-8 was identified as a significant inflammatory marker negatively associated with very long telomeres. CONCLUSION: These baseline findings bring new perspective to the relationship between air pollution and telomere length. Contrary to traditional views, the results suggest potential adaptive responses, highlighting the need for further longitudinal research to explore telomere dynamics over time in conjunction with other factors.

• Klíčová slova
• Air pollution, Inflammation, Lifestyle factors, Long-term exposure, Oxidative stress, Socioeconomic factors, Telomere length,
• MeSH
• benzen analýza   škodlivé účinky   MeSH
• benzopyren analýza   MeSH
• dospělí MeSH
• homeostáza telomer * MeSH
• kohortové studie MeSH
• látky znečišťující vzduch * analýza   škodlivé účinky   MeSH
• lidé středního věku MeSH
• lidé MeSH
• oxid dusičitý analýza   MeSH
• pevné částice analýza   škodlivé účinky   MeSH
• senioři MeSH
• telomery * účinky léků   MeSH
• vystavení vlivu životního prostředí * analýza   škodlivé účinky   MeSH
• životní styl MeSH
• znečištění ovzduší * škodlivé účinky   analýza   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• benzen MeSH
• benzopyren MeSH
• látky znečišťující vzduch * MeSH
• oxid dusičitý MeSH
• pevné částice MeSH

BACKGROUND: Asthma represents a syndrome for which our understanding of the molecular processes underlying discrete sub-diseases (i.e., endotypes), beyond atopic asthma, is limited. The public health needs to characterize etiology-associated endotype risks is becoming urgent. In particular, the roles of polyaromatic hydrocarbon (PAH), globally distributed combustion by-products, toward the two known endotypes - T helper 2 cell high (Th2) or T helper 2 cell low (non-Th2) - warrants clarification. OBJECTIVES: To explain ambient B[a]P association with non-atopic asthma (i.e., a proxy of non-Th2 endotype) is markedly different from that with atopic asthma (i.e., a proxy for Th2-high endotype). METHODS: In a case-control study, we compare the non-atopic as well as atopic asthmatic boys and girls against their respective controls in terms of the ambient Benzo[a]pyrene concentration nearest to their home, plasma 15-Ft2-isoprostane (15-Ft2-isoP), urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), and lung function deficit. We repeated the analysis for i) dichotomous asthma outcome and ii) multinomial asthma-overweight/obese (OV/OB) combined outcomes. RESULTS: The non-atopic asthma cases are associated with a significantly higher median B[a]P (11.16 ng/m3) compared to that in the non-atopic controls (3.83 ng/m3; P-value < 0.001). In asthma-OV/OB stratified analysis, the non-atopic girls with lean and OV/OB asthma are associated with a step-wisely elevated B[a]P (median,11.16 and 18.00 ng/m3, respectively), compared to the non-atopic lean control girls (median, 4.28 ng/m3, P-value < 0.001). In contrast, atopic asthmatic children (2.73 ng/m3) are not associated with a significantly elevated median B[a]P, compared to the atopic control children (2.60 ng/m3; P-value > 0.05). Based on the logistic regression model, on ln-unit increate in B[a]P is associated with 4.7-times greater odds (95% CI, 1.9-11.5, P = 0.001) of asthma among the non-atopic boys. The same unit increase in B[a]P is associated with 44.8-times greater odds (95% CI, 4.7-428.2, P = 0.001) among the non-atopic girls after adjusting for urinary Cotinine, lung function deficit, 15-Ft2-isoP, and 8-oxodG. CONCLUSIONS: Ambient B[a]P is robustly associated with non-atopic asthma, while it has no clear associations with atopic asthma among lean children. Furthermore, lung function deficit, 15-Ft2-isoP, and 8-oxodG are associated with profound alteration of B[a]P-asthma associations among the non-atopic children.

• Klíčová slova
• 8-oxo-7,8-dihydro-2′-deoxyguanosine, Air pollution, Benzo[a]pyrene, Endotype;15-Ft2-isoprostane,
• MeSH
• 8-hydroxy-2'-deoxyguanosin moč   MeSH
• benzopyren analýza   MeSH
• bronchiální astma krev   epidemiologie   patofyziologie   moč   MeSH
• dinoprost analogy a deriváty   krev   MeSH
• dítě MeSH
• fenotyp MeSH
• kojenec MeSH
• kotinin moč   MeSH
• látky znečišťující vzduch analýza   MeSH
• lidé MeSH
• mladiství MeSH
• plíce patofyziologie   MeSH
• předškolní dítě MeSH
• studie případů a kontrol MeSH
• vystavení vlivu životního prostředí analýza   MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH
• Názvy látek
• 8-epi-prostaglandin F2alpha MeSH Prohlížeč
• 8-hydroxy-2'-deoxyguanosin MeSH
• benzopyren MeSH
• dinoprost MeSH
• kotinin MeSH
• látky znečišťující vzduch MeSH

BACKGROUND: Within fossil- and solid-fuel dependent geographic locations, mechanisms of air pollution-induced asthma remains unknown. In particular, sources of greater genetic susceptibility to airborne carcinogen, namely, benzo[a]pyrene (B[a]P) has never been investigated beyond that of a few well known genes. OBJECTIVES: To deepen our understanding on how the genotypic variations within the candidate genes contribute to the variability in the children's susceptibility to ambient B[a]P on doctor-diagnosed asthma. METHODS: Clinically confirmed asthmatic versus healthy control children (aged, 7-15) were enrolled from historically polluted and rural background regions in Czech Republic. Contemporaneous ambient B[a]P concentration was obtained from the routine monitoring network. The sputum DNA was genotyped for 95 genes. B[a]P interaction with SNPs was studied by two-stage, semi-agnostic screening of 621 SNPs. RESULTS: The median B[a]P within the highly polluted urban center was 8-times higher than that in the background region (7.8 vs. 1.1 ng/m3) during the period of investigation. Within the baseline model, which considered B[a]P exposure-only, the second tertile range was associated with a significantly reduced odds (aOR = 0.28) of asthma (95% CI, 0.16 to 0.50) compared to those at the lowest range. However, the highest range of B[a]P was associated with 3.18-times greater odds of the outcome (95% CI, 1.77 to 5.71). Within the gene-environment interaction models, joint occurrence of a high B[a]P exposure range and having a high-risk genotype at CTLA4 gene (rs11571316) was associated with 9-times greater odds (95% CI, 4.56-18.36) of the asthma diagnosis. Similarly, rs11571319 at CTLA4 and a high B[a]P exposure range was associated with a 8-times greater odds (95% CI, 3.95-14.27) of asthma diagnosis. Furthermore, having TG + GG genotypes on rs1031509 near STAT4 was associated with 5-times (95% CI, 3.03-8.55) greater odds of asthma diagnosis at the highest B[a]P range, compared to the odds at the reference range. Also CYP2E1 AT + TT genotypes (rs2070673) was associated with 5-times (95% CI, 3.1-8.8) greater odds of asthma diagnosis at the highest B[a]P exposure. CONCLUSIONS: The children, who jointly experience a high B[a]P exposure (6.3-8.5 ng/m3) as well as susceptible genotypes in CTLA4 (rs11571316 and rs11571319), STAT4 (rs1031509), and CYP2E1 (rs2070673), respectively, are associated with a significantly greater odds of having doctor-diagnosed asthma, compared to those with neither risk factors.

• Klíčová slova
• Air pollution, Asthma, Gene-environment interaction, Polycyclic aromatic hydrocarbon, Single nucleotide polymorphism,
• MeSH
• antigen CTLA-4 genetika   MeSH
• benzopyren analýza   MeSH
• bronchiální astma chemicky indukované   genetika   MeSH
• cytochrom P-450 CYP2E1 genetika   MeSH
• dítě MeSH
• genetická predispozice k nemoci * MeSH
• genotyp MeSH
• interakce genů a prostředí MeSH
• jednonukleotidový polymorfismus MeSH
• lidé MeSH
• městské obyvatelstvo MeSH
• studie případů a kontrol MeSH
• transkripční faktor STAT4 genetika   MeSH
• venkovské obyvatelstvo MeSH
• vystavení vlivu životního prostředí analýza   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• znečištění ovzduší analýza   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• antigen CTLA-4 MeSH
• benzopyren MeSH
• CTLA4 protein, human MeSH Prohlížeč
• cytochrom P-450 CYP2E1 MeSH
• STAT4 protein, human MeSH Prohlížeč
• transkripční faktor STAT4 MeSH

This article is directed to determining concentrations of polycyclic aromatic hydrocarbons (PAHs), which are sorbed to solid particles in the air. Pollution sources were identified on the basis of the ratio of benzo[ghi]perylene (BghiPe) to benzo[a]pyrene (BaP). Because various important information is lost by determining the simple ratio of concentrations, least squares linear regression (classic ordinary least squares regression), reduced major axis, orthogonal regression, and Kendall-Theil robust diagnostics were utilized for identification. Statistical evaluation using all aforementioned methods demonstrated different ratios of the monitored PAHs in the intervals examined during warmer and colder periods. Analogous outputs were provided by comparing gradients of the emission factors acquired from the measured concentrations of BghiPe and BaP in motor vehicle exhaust gases. Based on these outputs, it was possible plausibly to state that the influence of burning organic fuels in heating stoves is prevalent in colder periods whereas in warmer periods transport was the exclusive source because other sources of PAH emissions were not found in the examined locations.

• Klíčová slova
• Air pollution, Benzo[a]pyrene, Benzo[ghi]perylene, Pollution sources, Polycyclic aromatic hydrocarbons, Regression, Transport,
• MeSH
• benzopyren analýza   MeSH
• hodnocení rizik MeSH
• látky znečišťující vzduch analýza   MeSH
• lineární modely MeSH
• metoda nejmenších čtverců MeSH
• monitorování životního prostředí metody   statistika a číselné údaje   MeSH
• perylen analogy a deriváty   analýza   MeSH
• pevné částice analýza   MeSH
• polycyklické aromatické uhlovodíky analýza   MeSH
• roční období MeSH
• urbanizace MeSH
• výfukové emise vozidel analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• 1,12-benzoperylene MeSH Prohlížeč
• benzopyren MeSH
• látky znečišťující vzduch MeSH
• perylen MeSH
• pevné částice MeSH
• polycyklické aromatické uhlovodíky MeSH
• výfukové emise vozidel MeSH

The Northern Moravia Region is the most polluted region in the Czech Republic by particulate matter (PM2.5) and carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) as benzo[a]pyrene (B[a]P) by heavy industry and local heating. This specific situation was used to study the impact of air pollution on newborns in the exposed Karviná district and control district of České Budějovice. Biological material from newborns and mothers was collected in summer and winter seasons. This project is highly detailed, analyzing the concentrations of PAHs in ambient air and diet, in human breast milk, in the urine of mothers and newborns, using biomarkers of genetic damage as DNA adducts and gene expression analysis, biomarkers of oxidative stress as 8-oxodG adducts and lipid peroxidation (15-F2t-isoprostane immunoassay). All 400 children, for whom the biomarker data at delivery were obtained, will be followed for morbidity up to 2 years of age. The Northern Moravia Region seems to be to be a model area for studying the long-term impact of human health exposure to c-PAHs. Our observations will indicate possible genetic and oxidative damage in newborns, which may significantly affect their morbidity.

• Klíčová slova
• air pollution, diet, genetic damage, molecular epidemiology, newborns, oxidative stress, polycyclic aromatic hydrocarbons,
• MeSH
• 8-hydroxy-2'-deoxyguanosin MeSH
• adukty DNA MeSH
• benzopyren analýza   MeSH
• deoxyguanosin analogy a deriváty   analýza   MeSH
• dieta MeSH
• dospělí MeSH
• genom * MeSH
• kvantitativní polymerázová řetězová reakce MeSH
• látky znečišťující vzduch analýza   MeSH
• lidé MeSH
• mateřské mléko chemie   MeSH
• monitorování životního prostředí MeSH
• novorozenec MeSH
• oxidační stres MeSH
• pevné částice analýza   MeSH
• polycyklické aromatické uhlovodíky analýza   MeSH
• průmysl MeSH
• roční období MeSH
• těhotenství MeSH
• vystavení vlivu životního prostředí analýza   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• 8-hydroxy-2'-deoxyguanosin MeSH
• adukty DNA MeSH
• benzopyren MeSH
• deoxyguanosin MeSH
• látky znečišťující vzduch MeSH
• pevné částice MeSH
• polycyklické aromatické uhlovodíky MeSH

This study estimated current benzo(a)pyrene (BaP) concentration levels, population exposure and potential health impacts of exposure to ambient air BaP in Europe. These estimates were done by combining the best available information from observations and chemical transport models through the use of spatial interpolation methods. Results show large exceedances of the European target value for BaP in 2012 over large areas, particularly in central-eastern Europe. Results also show large uncertainties in the concentration estimates in regions with a few or no measurement stations. The estimation of the population exposure to BaP concentrations and its health impacts was limited to 60% of the European population, covering only the modelled areas which met the data quality requirement for modelling of BaP concentrations set by the European directive 2004/107/EC. The population exposure estimate shows that 20% of the European population is exposed to BaP background ambient concentrations above the EU target value and only 7% live in areas with concentrations under the estimated acceptable risk level of 0.12 ng m(-3). This exposure leads to an estimated 370 lung cancer incidences per year, for the 60% of the European population included in the estimation. Emissions of BaP have increased in the last decade with the increase in emissions from household combustion of biomass. At the same time, climate mitigation policies are promoting the use of biomass burning for domestic heating. The current study shows that there is a need for more BaP measurements in areas of low measurement density, particularly where high concentrations are expected, e.g. in Romania, Bulgaria, and other Balkan states. Furthermore, this study shows that the health risk posed by PAH exposure calls for better coordination between air quality and climate mitigation policies in Europe.

• Klíčová slova
• Benzo(a)pyrene, Health effects, Kriging, Polycyclic Aromatic Hydrocarbons, Population exposure,
• MeSH
• benzopyren * škodlivé účinky   analýza   MeSH
• biomasa MeSH
• incidence MeSH
• látky znečišťující vzduch * škodlivé účinky   analýza   MeSH
• lidé MeSH
• nádory plic epidemiologie   etiologie   MeSH
• rizikové faktory MeSH
• vystavení vlivu životního prostředí * škodlivé účinky   analýza   MeSH
• vytápění MeSH
• znečištění ovzduší * škodlivé účinky   analýza   MeSH
• znečištění vzduchu ve vnitřním prostředí škodlivé účinky   analýza   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Evropa epidemiologie   MeSH
• východní Evropa epidemiologie   MeSH
• Názvy látek
• benzopyren * MeSH
• látky znečišťující vzduch * MeSH

A surface plasmon resonance (SPR) biosensor for simultaneous detection of multiple organic pollutants exhibiting endocrine-disrupting activity, namely atrazine, benzo[a]pyrene, 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-nonylphenol, is reported. The biosensor utilizes a multichannel SPR sensor based on wavelength modulation of SPR and wavelength division multiplexing (WDM) of sensing channels, antibodies as biorecognition element and a competitive immunoassay detection format. An analysis time of 45 min (including 30-min incubation of the sample with antibodies) and limits of detection as low as 0.05, 0.07, 0.16 and 0.26 ng mL(-1) are demonstrated for benzo[a]pyrene, atrazine, 2,4-D and 4-nonylphenol, respectively. The biosensor is also shown to be regenerable and suitable for repeated use.

• MeSH
• atrazin analýza   MeSH
• benzopyren analýza   MeSH
• biosenzitivní techniky přístrojové vybavení   metody   MeSH
• časové faktory MeSH
• endokrinní disruptory analýza   MeSH
• fenoly analýza   MeSH
• imunoanalýza metody   MeSH
• kyselina 2,4-dichlorfenoxyoctová analýza   MeSH
• povrchová plasmonová rezonance přístrojové vybavení   metody   MeSH
• senzitivita a specificita MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 4-nonylphenol MeSH Prohlížeč
• atrazin MeSH
• benzopyren MeSH
• endokrinní disruptory MeSH
• fenoly MeSH
• kyselina 2,4-dichlorfenoxyoctová MeSH

The effect of exposure to organic compounds adsorbed onto respirable air particles (<2.5microm) on DNA adducts in lymphocytes was studied in a group of non-smoking policemen (N=109, aged 35+/-0.9 years) working in the downtown area of Prague and spending >8h daily outdoors. Personal exposure to carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) adsorbed on respirable particles was monitored in each subject for 48h before biological sampling. DNA adducts were analyzed by a (32)P-postlabelling assay, and total DNA adduct levels and B[a]P-like spots were determined. Further biomarkers included cotinine levels in urine to control for exposure to tobacco smoke, plasma levels of vitamins A, E and C and polymorphisms of metabolic genotypes (GSTM1, GSTP1, GSTT1, CYP 1A1-Msp I and Ile/Val, MTHFR, MS), DNA repair genotypes (XRCC1, hOGG1 and XPD exons 6 and 23) and the p53 gene (p53 Msp I and BstU I). All the biomarkers of exposure and effect were analyzed repeatedly during a period of one year at 2-3 month intervals (January, March, June, September 2004) to cover periods with high (winter) and low (summer) levels of air pollution. The highest personal exposure to c-PAHs was found in January (8.1+/-8.8ng/m(3)), while the other three sampling periods exhibited 3-4-fold lower c-PAH exposure. The total DNA adducts were only slightly elevated in January (2.08+/-1.60) compared to March (1.66+/-0.65), June (1.96+/-1.73) and September (1.77+/-1.77). B[a]P-like DNA adducts, however, were significantly higher in January than in the March and June sampling periods (0.26+/-0.14 vs. 0.19+/-0.12 and 0.22+/-0.13, respectively; p<0.0001 and p=0.017) indicating that c-PAH exposure probably plays a crucial role in DNA adduct formation in lymphocytes. No effect of individual metabololic or DNA repair genotypes on DNA adduct levels was observed. However, the combination of two genotypes encoding enzymes metabolizing c-PAHs - CYP 1A1 and GSTM1 - was associated with the levels of total and B[a]P-like DNA adducts under conditions of increased exposure to c-PAHs. Our study suggests that DNA adducts in the lymphocytes of subjects exposed to increased c-PAH levels are an appropriate biomarker of a biologically effective dose, directly indicating whether or not the extent of exposure to these compounds is related to an increased mutagenic and carcinogenic risk.

• MeSH
• adukty DNA analýza   MeSH
• benzopyren analýza   toxicita   MeSH
• biologické markery analýza   MeSH
• dospělí MeSH
• genotyp MeSH
• karcinogeny životního prostředí analýza   toxicita   MeSH
• látky znečišťující vzduch v pracovním prostředí analýza   toxicita   MeSH
• látky znečišťující vzduch analýza   toxicita   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lymfocyty chemie   účinky léků   MeSH
• mutageny analýza   toxicita   MeSH
• policie * MeSH
• polycyklické aromatické uhlovodíky analýza   toxicita   MeSH
• polymorfismus genetický MeSH
• pracovní expozice * MeSH
• roční období MeSH
• znečištění ovzduší škodlivé účinky   analýza   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• adukty DNA MeSH
• benzo(a)pyrene-DNA adduct MeSH Prohlížeč
• benzopyren MeSH
• biologické markery MeSH
• karcinogeny životního prostředí MeSH
• látky znečišťující vzduch v pracovním prostředí MeSH
• látky znečišťující vzduch MeSH
• mutageny MeSH
• polycyclic aromatic hydrocarbons-DNA adduct MeSH Prohlížeč
• polycyklické aromatické uhlovodíky MeSH

Organic pollution is a complex mixture where besides usually discussed polycyclic aromatic hydrocarbons (PAHs) a lot of other toxic or potentially toxic compounds occur. In this case, the organic air pollution in two important industrial cities, Sarajevo and Tuzla, in Bosnia and Herzegovina (part of former Yugoslavia) was assessed with the emphasis placed on genotoxic risks using both chemical (PAHs analyses) and biological approaches (genotoxicity testing with a screening bacterial genotoxicity test - SOS chromotest). The study was performed as a part of the APOPSBAL project (ICA2-CT2002-10007). So far there has not been any information either about the PAHs pollution or the genotoxic activity of the organic air pollution for the localities under the study. Therefore, the presented information is considered absolutely unique. Both used approaches made possible to identify the localities with the highest pollution level and genotoxic risks in both cities. Generally, higher levels of both parameters were determined in Tuzla, which is much more industrialized than Sarajevo, and especially at localities close to city centers and affected by traffic emissions, but also at localities polluted by emissions from industry and household heating. Even if benzo(a)pyrene concentrations exceeded the maximum permitted levels for this pollutant at some localities in Tuzla, the PAHs concentrations were fully comparable with the levels determined in other industrial European cities. Significant genotoxicity of the organic extracts was detected for almost all of the urban localities in the test both without (-S9; direct genotoxicity) and with the addition of metabolic activation (+S9; indirect genotoxicity). The observed direct genotoxic activities were discussed in relation to a potential presence of PAHs derivatives in the air. The indirect genotoxic activities were apparently higher at the localities with higher contents of carcinogenic PAHs. The significant relationship between the determined genotoxic activities and the PAHs pollution was also confirmed by a regression analysis. However, the correlations were not absolute because the observed genotoxic activity was also dependent on the presence of other organic pollutants than the PAHs. It concerns predominantly direct genotoxicity which is not related with the PAHs, but with their nitro-, oxi-, and hydroxy-derivatives and also other unknown polar organic pollutants. However, the concentrations of the direct genotoxins apparently correlated with the PAHs contents in the air. The study showed that screening genotoxicity tests, such as the SOS chromotest, could be effectively used for the identification of localities with increased genotoxic risks. In comparison with the health risk assessment which is usually based on the chemical analyses of only a small part of the pollution mixture, the bioassays enable us to evaluate the risks of all the mixture. The localities with the highest detected human health risks according to the screening bioassays may then be analyzed in detail with specific chemical methods to identify their causes.

• MeSH
• benzopyren analýza   MeSH
• hodnocení rizik MeSH
• monitorování životního prostředí * MeSH
• polycyklické aromatické uhlovodíky analýza   MeSH
• regresní analýza MeSH
• testy genotoxicity MeSH
• velkoměsta MeSH
• znečištění ovzduší analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Bosna a Hercegovina MeSH
• velkoměsta MeSH
• Názvy látek
• benzopyren MeSH
• polycyklické aromatické uhlovodíky MeSH

Acellular assay of calf thymus DNA+/-rat liver microsomal S9 fraction coupled with (32)P-postlabelling was used to study the genotoxic potential of organic compounds bound onto PM10 particles collected in three European cities-Prague (CZ), Kosice (SK) and Sofia (BG) during summer and winter periods. B[a]P alone induced DNA adduct levels ranging from 4.8 to 768 adducts/10(8) nucleotides in the concentration dependent manner. However, a mixture of 8 c-PAHs with equimolar doses of B[a]P induced 3.7-757 adducts/10(8) nucleotides, thus suggesting the inhibition of DNA adduct forming activity by interaction among various PAHs. Comparison of DNA adduct levels induced by various EOMs indicates higher variability among seasons than among localities. DNA adduct levels for Prague collection site varied from 19 to 166 adducts/10(8) nucleotides, for Kosice from 22 to 85 and for Sofia from 6 to 144 adducts/10(8) nucleotides. Bioactivation with S9 microsomal fraction caused 2- to 7-fold increase in DNA adduct levels compared to -S9 samples, suggesting a crucial role of indirectly acting genotoxic EOM components, such as PAHs. We have demonstrated for the first time a significant positive correlation between B[a]P content in EOMs and total DNA adduct levels detected in the EOM treated samples (R=0.83; p=0.04). These results suggest that B[a]P content in EOM is an important factor for the total genotoxic potential of EOM and/or B[a]P is a good indicator of the presence of other genotoxic compounds causing DNA adducts. Even stronger correlation between the content of genotoxic compounds in EOMs and total DNA adduct levels detected (R=0.94; p=0.005) was found when eight c-PAHs were taken into the consideration. Our findings support a hypothesis that a relatively limited number of EOM components is responsible for a major part of its genotoxicity detectable as DNA adducts by (32)P-postlabelling.

• MeSH
• adukty DNA analýza   MeSH
• benzopyren analýza   MeSH
• karcinogeny životního prostředí toxicita   MeSH
• krysa rodu Rattus MeSH
• látky znečišťující vzduch toxicita   MeSH
• lidé MeSH
• organické látky toxicita   MeSH
• pevné částice toxicita   MeSH
• polycyklické aromatické uhlovodíky metabolismus   toxicita   MeSH
• testy genotoxicity metody   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adukty DNA MeSH
• benzo(a)pyrene-DNA adduct MeSH Prohlížeč
• benzopyren MeSH
• karcinogeny životního prostředí MeSH
• látky znečišťující vzduch MeSH
• organické látky MeSH
• pevné částice MeSH
• polycyklické aromatické uhlovodíky MeSH