Vibrio alginolyticus is a Gram-negative halophilic bacterium and has been recognized as an opportunistic pathogen to both humans and marine animals. So far, most studies have been focused on marine animals and few reports have been aimed at mammals, including human. In this study, we first established a mouse model to understand the pathogenic mechanism of V. alginolyticus infection. After infection via intraperitoneal injection, hematological and liver function indicators were evaluated and serum interleukin (IL)-1β and IL-6 expression were detected by ELISA. Furthermore, we compared the virulence of two V. alginolyticus strains, ATCC17749T and E0666. The results demonstrated that V. alginolyticus infection causes robust lung and liver damage and induces changes in IL-1β, IL-6, hematological, and liver indicators. In addition, the ATCC17749T strain appeared to be more virulent than the E0666 strain. Better understanding of the pathogenic mechanism of V. alginolyticus infection should guide effective prevention and therapy for V. alginolyticus infection.

• MeSH
• biochemická analýza krve MeSH
• cytokiny krev   MeSH
• enzymy krev   MeSH
• histocytochemie MeSH
• infekce bakteriemi rodu Vibrio mikrobiologie   patologie   MeSH
• jaterní testy MeSH
• játra patologie   MeSH
• mikroskopie MeSH
• modely nemocí na zvířatech MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• plíce patologie   MeSH
• Vibrio alginolyticus patogenita   MeSH
• virulence MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• cytokiny MeSH
• enzymy MeSH

OBJECTIVE: In the pathogenesis of the metabolic syndrome (MetS), an increase of oxidative stress could play an important role which is closely linked with insulin resistance, endothelial dysfunction, and chronic inflammation. The aim of our study was to assess several parameters of the antioxidant status in MetS. METHODS: 40 subjects with MetS and 40 age- and sex-matched volunteers without MetS were examined for activities of superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase 1 (GPX1), glutathione reductase (GR), paraoxonase1 (PON1), concentrations of reduced glutathione (GSH), and conjugated dienes in low-density lipoprotein (CD-LDL). RESULTS: Subjects with MetS had higher activities of CuZnSOD (p < 0.05) and GR (p < 0.001), higher concentrations of CD-LDL (p < 0.001), lower activities of CAT (p < 0.05) and PON1 (p < 0.05), and lower concentrations of GSH (p < 0.05), as compared with controls. Activity of GPX1 was not significantly changed. CONCLUSIONS: Our results implicated an increased oxidative stress in MetS and a decreased antioxidative defense that correlated with some laboratory (triglycerides, high-density lipoprotein cholesterol (HDL-C)) and clinical (waist circumference, blood pressure) components of MetS.

• MeSH
• antioxidancia analýza   MeSH
• aryldialkylfosfatasa krev   MeSH
• biologické markery krev   MeSH
• enzymy krev   MeSH
• glutathion krev   MeSH
• glutathionperoxidasa (GPx1) MeSH
• glutathionperoxidasa krev   MeSH
• glutathionreduktasa krev   MeSH
• katalasa krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lipoproteiny LDL krev   MeSH
• metabolický syndrom krev   diagnóza   enzymologie   MeSH
• oxidační stres MeSH
• studie případů a kontrol MeSH
• superoxiddismutasa krev   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• aryldialkylfosfatasa MeSH
• biologické markery MeSH
• enzymy MeSH
• glutathion MeSH
• glutathionperoxidasa (GPx1) MeSH
• glutathionperoxidasa MeSH
• glutathionreduktasa MeSH
• GPX1 protein, human MeSH Prohlížeč
• katalasa MeSH
• lipoproteiny LDL MeSH
• PON1 protein, human MeSH Prohlížeč
• superoxiddismutasa MeSH

Alcohol use has been identified as a risk factor for the development of osteoporosis. Eight male Wistar rats at two months of age were alcoho-fed (7.6 g 95 % ethanol/kg b.w. per day) to evaluate the effects of long-term administration (three months) of alcohol in drinking water. We have used a dose which is considered to be comparable to a dose of 1 liter of wine or 2.5 liters of 12(°) beer used in male adults daily. The bones were tested mechanically by a three-point bending test in a Mini Bionix (MTS) testing system. The bones from alcohol-fed rats were characterized by a reduction in bone density as well as in ash, calcium and phosphate content. In alcohol-fed rats the reduction in bone mineral density (10 %) was reflected by about 12 % reduction of mechanical strength of femur (158+/-5.5 vs. 178+/-3.2 N/mm(2)). Alcohol significantly altered femoral cortical thickness. In our experiment alcohol itself did not exert any antiandrogenic effect and it did not produce changes in the weight of seminal vesicles. Liver function test (GGT, ALP, AST) did not differ between alcohol-fed rats and control rats. Alcohol-induced bone loss is associated with increased bone resorption and decreased bone formation. These results document the efficacy of alcohol at the dose of 7.6 g 95 % ethanol/kg b.w. to cause bone loss and loss of bone mechanical strength in intact rats. The results of the present study may be interpreted as supporting the hypothesis of alcohol as a risk factor for osteoporosis.

• MeSH
• biologické markery krev   MeSH
• biomechanika MeSH
• časové faktory MeSH
• enzymy krev   MeSH
• ethanol aplikace a dávkování   toxicita   MeSH
• femur účinky léků   metabolismus   patologie   MeSH
• fosfáty krev   MeSH
• jaterní testy MeSH
• játra účinky léků   enzymologie   MeSH
• kostní denzita účinky léků   MeSH
• krysa rodu Rattus MeSH
• osteogeneze účinky léků   MeSH
• osteoporóza chemicky indukované   metabolismus   patologie   MeSH
• pití alkoholu škodlivé účinky   MeSH
• potkani Wistar MeSH
• resorpce kosti chemicky indukované   metabolismus   patologie   MeSH
• semenné váčky účinky léků   MeSH
• vápník krev   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH
• enzymy MeSH
• ethanol MeSH
• fosfáty MeSH
• vápník MeSH

OBJECTIVES: To investigate the activities of the main antioxidative enzymes and oxidative stress in women with depressive disorder (DD). METHODS: In 35 drug-naive women with DD and 35 age matched healthy women enzymes superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPX1), glutathione reductase (GR) and paraoxonase (PON1), concentrations of conjugated dienes (CD), reduced glutathione (GSH) and anthropometric and clinical data were investigated. RESULTS: Women with DD were found to have decreased activities of GPX1 (p<0.05), decreased concentrations of GSH (p<0.05), and increased activities of GR (p<0.05), CuZnSOD (p<0.001), and concentrations of CD (p<0.05). Activity of GPX1 was positively correlated with concentration of GSH (p<0.05). Concentrations of CD were positively correlated with TG (p<0.01). CONCLUSION: Our set of depressive women was characterized by changes indicating an increased oxidative stress, as well as by certain features of metabolic syndrome.

• MeSH
• alkadieny krev   chemie   MeSH
• aryldialkylfosfatasa krev   metabolismus   MeSH
• depresivní poruchy krev   enzymologie   patologie   MeSH
• enzymy krev   metabolismus   MeSH
• glutathion krev   metabolismus   MeSH
• glutathionperoxidasa krev   metabolismus   MeSH
• glutathionreduktasa krev   metabolismus   MeSH
• katalasa krev   metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• oxidace-redukce MeSH
• oxidační stres * MeSH
• senioři MeSH
• spektrofotometrie MeSH
• superoxiddismutasa krev   metabolismus   MeSH
• triglyceridy krev   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alkadieny MeSH
• aryldialkylfosfatasa MeSH
• enzymy MeSH
• glutathion MeSH
• glutathionperoxidasa MeSH
• glutathionreduktasa MeSH
• katalasa MeSH
• PON1 protein, human MeSH Prohlížeč
• superoxiddismutasa MeSH
• triglyceridy MeSH

Recently, pyridoxal 2-chlorobenzoyl hydrazone (o-108) has been identified as an effective iron chelator [Link et al., Blood 2003; 101: 4172-79]. Since chronic treatment would be necessary in its potential indications, in the present study, the safety and tolerability of this agent after repeated administration was determined. Three doses of o-108 (25, 50, 100 mg/kg, in 10% Cremophor EL) were administered intraperitoneally, once weekly, for 10 weeks to three groups (n=5 each) of Chinchilla male rabbits. The effects on biochemical, haematological and cardiovascular parameters were examined during the experiment; histopathological examination was performed at the end of the experiment. Results were compared with control (saline 2 mL/kg, n=11) and vehicle groups (10% Cremophor EL, 2 mL/kg, n=12). No premature deaths occurred; the well-being of animals was evidenced by their body weight gain, although lower gain was observed with the highest dose (100 mg/kg). Significant elevations of cardiac troponin T plasma concentrations were observed with the highest dose of o-108, but no abnormalities were found in the cardiovascular function and only minor and inconsistent changes in haematological and biochemical parameters were observed. Histopathological examinations of selected organs revealed only weak and reversible changes through all studied groups. Thus, the data from this study suggest that o-108 remains a promising drug from the standpoint of the possibility of its repeated administration and warrants further investigation.

• MeSH
• časové faktory MeSH
• chelátory železa aplikace a dávkování   toxicita   MeSH
• enzymy krev   MeSH
• hydrazony aplikace a dávkování   farmakokinetika   toxicita   MeSH
• králíci MeSH
• krevní obraz MeSH
• mikroskopie elektronová rastrovací MeSH
• pyridoxal aplikace a dávkování   analogy a deriváty   farmakokinetika   toxicita   MeSH
• tělesná hmotnost účinky léků   MeSH
• tkáňová distribuce MeSH
• troponin T krev   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• chelátory železa MeSH
• enzymy MeSH
• hydrazony MeSH
• pyridoxal 2-chlorobenzoyl hydrazone MeSH Prohlížeč
• pyridoxal MeSH
• troponin T MeSH

Rainbow trout at a weight of 223+/-12 g (mean+/-SD) were experimentally injected with a technical mixture of Delor 103 to evaluate the red blood cell indices (red blood cell count, haematocrit, haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration) and some biochemical and enzyme parameters of the blood plasma (total protein, glucose, inorganic phosphate, total calcium, sodium, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase). Delor 103, administered by the i.p. route at a concentration of 0.24 g kg(-1) 120 h(-1), caused an increase in the red blood cell counts, haematocrit values, haemoglobin concentrations, inorganic phosphate, alanine aminotransferase and lactate dehydrogenase. The sodium level fell. The fish injected with Delor 103 showed a relative decrease in the lymphocyte count and a relative increase in the count of neutrophile band forms.

• MeSH
• aktivace enzymů účinky léků   MeSH
• biochemická analýza krve veterinární   MeSH
• enzymy krev   MeSH
• erytrocytární znaky účinky léků   MeSH
• fosfáty krev   MeSH
• hematokrit veterinární   MeSH
• hematologické testy veterinární   MeSH
• hemoglobiny metabolismus   MeSH
• krevní glukóza MeSH
• krevní proteiny MeSH
• lymfocyty účinky léků   MeSH
• Oncorhynchus mykiss metabolismus   MeSH
• počet erytrocytů veterinární   MeSH
• polychlorované bifenyly toxicita   MeSH
• sodík krev   MeSH
• testy genotoxicity metody   veterinární   MeSH
• vápník krev   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• 2,4',5-trichlorobiphenyl MeSH Prohlížeč
• enzymy MeSH
• fosfáty MeSH
• hemoglobiny MeSH
• krevní glukóza MeSH
• krevní proteiny MeSH
• polychlorované bifenyly MeSH
• sodík MeSH
• vápník MeSH

Soman belongs to the most dangerous nerve agents because of the low effectiveness of the presently available antidotes. Soman acts by inhibiting acetylcholinesterase (AChE) both peripherally and centrally, with a subsequent accumulation of neuromediator acetylcholine and other metabolic changes. From the data published in literature it can be concluded that exposure to nerve agents leading to acute effects or chronic exposure to nerve agents may lead to delayed and persistent adverse effects. The aim of this study was to demonstrate changes in AChE and butyrylcholinesterase (BuChE) activities, stressogenic markers (i.e., tyrosine aminotransferase [TAT] activity, and plasma corticosterone level), and neuroexcitability and behavior 24 h and 4 wk following a single soman inhalation exposure at low level. AChE activity in erythrocytes and BuChE activity in plasma was decreased (dependent on the dose of soman) 24 h and 4 wk after the exposure. A similar decrease in AChE activity in different brain parts was observed. One of the stressogenic parameters, TAT, was changed 24 h after exposure only. Behavior of experimental animals was changed 24 h after the exposure, and 4 behavioral parameters persisted 4 wk after the exposure. Neuroexcitability was increased at 24 h after the exposure and had become about normal 4 wk after the exposure. Summarizing, long-term effects (4 wk) were observed after inhalation exposure of guinea pigs to sublethal concentrations of soman.

• MeSH
• acetylcholinesterasa krev   MeSH
• aplikace inhalační MeSH
• butyrylcholinesterasa krev   MeSH
• chemické bojové látky toxicita   MeSH
• cholinesterasové inhibitory aplikace a dávkování   toxicita   MeSH
• cholinesterasy krev   MeSH
• chování zvířat účinky léků   MeSH
• enzymy krev   MeSH
• erytrocyty účinky léků   enzymologie   MeSH
• inhalační expozice MeSH
• kortikosteron krev   MeSH
• morčata MeSH
• mozek účinky léků   enzymologie   MeSH
• soman aplikace a dávkování   toxicita   MeSH
• tyrosinaminotransferasa krev   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• morčata MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• acetylcholinesterasa MeSH
• butyrylcholinesterasa MeSH
• chemické bojové látky MeSH
• cholinesterasové inhibitory MeSH
• cholinesterasy MeSH
• enzymy MeSH
• kortikosteron MeSH
• soman MeSH
• tyrosinaminotransferasa MeSH

Troponin T (TnT) is recently being considered to be an important diagnostic marker of myocardial damage in adults, but this marker has not yet been used in neonates. The present study was designed to determine the normal level of cardiac TnT in the cord blood of healthy term neonates. Cardiac troponin T concentration in cord blood was measured in 15 healthy term neonates using commercial kit (Enzymun-Test System, Boehringer, Mannheim). TnT serum concentration was 0.05 +/- 0.04 micrograms/l in 10 of 15 babies whereas in the remaining 5 haemolysed samples its concentration was elevated (mean 0.19 +/- 0.07 micrograms/l). It is important to consider that incidental haemolysis of blood samples can mimic pathological elevation of TnT by interfering with the assay.

• MeSH
• biochemická analýza krve MeSH
• biologické markery MeSH
• enzymy krev   MeSH
• fetální krev chemie   MeSH
• lidé MeSH
• novorozenec MeSH
• referenční hodnoty MeSH
• tělesná hmotnost MeSH
• troponin T MeSH
• troponin krev   MeSH
• věkové faktory MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH
• enzymy MeSH
• troponin T MeSH
• troponin MeSH

Chronic toxic effects of supermethrin on some biochemical parameters (AST, ALT, LDH, creatinine and total proteins) were investigated in 84 individuals of Japanese quail divided into four groups (control-K, experimental group I-P1, experimental group II-P2, experimental group III-P3) in the conditions of 140-day avian reproductive test. The three experimental groups received the tested substance at these doses: P1-10.7 mg/kg l.w./day, P2-21.4 mg/kg l.w./day, P3-35.7 mg/kg l.w./day. The results of observation of the enzyme activities AST and ALT show that only the AST activity (in the course of 140-day avian reproductive test) significantly increased to 1.225 mu kat/l in the females of experimental group P1, to 1.053 mu kat/l in P2 and to 1.014 mu kat/l in P3 against the control, in which the AST activity was 0.670 mu kat/l. The values of AST activity in the males were 1.143 mu kat/l in P1, 1.117 mu kat/l in P2 and 1.090 mu kat/l in P3 against the control 0.8395 mu kat/l. The investigation of variations in total LDH activity in Japanese quail after 140-day avian reproductive test has shown an increase in the LDH activity in the males (11.193 mu kat/l in P1, 11.269 mu kat/l in P2, 8.245 mu kat/l in P3 and 7.362 mu kat/l in K) as well as in the females (10.91 mu kat/l in P1, 12.023 mu kat/l in P2, 10.196 mu kat/l in P3 and 7.055 mu kat/l in K).(ABSTRACT TRUNCATED AT 250 WORDS)

• MeSH
• Coturnix fyziologie   MeSH
• enzymy krev   MeSH
• insekticidy toxicita   MeSH
• pyrethriny toxicita   MeSH
• rozmnožování účinky léků   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• cypermethrin MeSH Prohlížeč
• enzymy MeSH
• insekticidy MeSH
• pyrethriny MeSH

The study discusses the basic quantitative indices used as a standard method in foreign professional literature dealing with paternity cases. They are as follows: 1. mean probability of exclusion (PE) which characterizes the informative value of the experts opinions and is the same in all the disputes evaluated by this expert. 2. relative frequency of men chosen at random from the population and excluded at given phenotype of mother and child (RME). 3. probability of paternity (PP) for particular trio: mother-child-the accused man. Hereinafter the results of our studies in the HLA laboratory in Olomouc from 1976-1991 are introduced.

• MeSH
• antigeny krevních skupin krev   MeSH
• dítě MeSH
• enzymy krev   MeSH
• haplotypy MeSH
• HLA antigeny krev   MeSH
• krevní proteiny analýza   MeSH
• lidé MeSH
• matky MeSH
• otcové MeSH
• paternita * MeSH
• pravděpodobnost MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny krevních skupin MeSH
• enzymy MeSH
• HLA antigeny MeSH
• krevní proteiny MeSH