UVA photons are less energetic than UVB photons but they are more abundant in solar radiation. Modern tools have shown that UVA light has serious adverse effects on the skin. We investigated the effect of consuming Lonicera caerulea berries on UVA-induced damage in SKH-1 mice. The mice were fed a diet containing L. caerulea berries (10%, w/w) for 14 days before a single UVA (30 J/cm(2)) treatment. Effects on haematological and antioxidant parameters were evaluated 4 and 24h after irradiation. The bioavailability of L. caerulea phenolics was also assessed. Consuming the L. caerulea berry-enriched diet caused reduced malondialdehyde production and increased catalase activity and glutathione levels were found in skin and erythrocytes. UVA-induced NADPH:quinone oxidoreductase-1 and gamma-L-glutamate-L-cysteine ligase protein in skin were reduced in mice fed L. caerulea berries. Enhanced heme oxygenase-1 level in skin, interleukin-17 in plasma and reduced interleukin-12 levels in plasma were found in the mice on the experimental diet. Histological (pyknotic) changes in the nuclei of basal cells induced by UVA exposure were reduced in L. caerulea berry consuming animals. HLPC-MS analysis showed high concentrations of hippuric acid, one of the main metabolites of aromatic amino acids and phenolic compounds, in skin, liver, urine and faeces of mice consuming the berries. Taken together, consumption of L. caerulea berries affords protection from the adverse effects of a single UVA exposure mainly via modulation of antioxidant parameters.
- Klíčová slova
- Blue honeysuckle, Erythrocyte, Hairless mice, Liver, Skin, UVA radiation,
- MeSH
- antioxidancia metabolismus MeSH
- dieta * MeSH
- enzymy metabolismus MeSH
- erytrocyty metabolismus účinky záření MeSH
- glutathion metabolismus MeSH
- hippuráty analýza moč MeSH
- interleukin-12 krev MeSH
- interleukin-17 krev MeSH
- játra chemie MeSH
- kůže metabolismus patologie účinky záření MeSH
- Lonicera chemie metabolismus MeSH
- malondialdehyd metabolismus MeSH
- myši bezsrsté MeSH
- myši MeSH
- ovoce chemie metabolismus MeSH
- ultrafialové záření * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- enzymy MeSH
- glutathion MeSH
- hippuráty MeSH
- hippuric acid MeSH Prohlížeč
- interleukin-12 MeSH
- interleukin-17 MeSH
- malondialdehyd MeSH
BACKGROUND: The Goeckerman regimen (GR) involves the dermal application of a crude coal tar (polycyclic aromatic hydrocarbon, PAH) and exposure to ultraviolet (UV) radiation. Both PAH and UV radiation exhibit immunosuppressive activity. This study describes the changes in the serum levels of the pro-inflammatory cytokine interleukin-12 (IL-12) and the anti-inflammatory cytokine IL-10 in patients with psoriasis (n = 55) treated with GR. METHODS: The serum levels of IL-12 and IL-10 were compared before and after GR. In addition, the IL-12 and IL-10 levels in psoriatic patients were compared with those in a control group of healthy blood donors (n = 47). The Psoriasis Area and Severity Index (PASI) was used to evaluate the efficacy of GR. RESULTS: When compared with the control group, both IL-12 and IL-10 were significantly higher in psoriatic patients in all cases (P < 0.001). When compared before and after GR, the IL-12 and IL-10 levels (P < 0.01) and PASI value (P < 0.001) were significantly lower after GR. The decrease in the serum level of IL-12 and IL-10 after GR was related to the entry value before GR (IL-12, r = 0.60, P < 0.001; IL-10, r = 0.36, P < 0.01). There was a significant correlation between the IL-10 level before GR and the PASI value after GR (r = -0.39; P < 0.01). CONCLUSIONS: The results indicate a strong pro-inflammatory effect of IL-12 in the immunopathogenesis of psoriasis, and confirm the immunosuppressive and anti-inflammatory effect of GR. IL-10 seems to be a promising individual marker for a positive effect of GR therapy.
- MeSH
- aplikace lokální MeSH
- biologické markery analýza krev MeSH
- cytokiny krev MeSH
- dehet uhelný terapeutické užití MeSH
- dospělí MeSH
- ELISA MeSH
- hodnocení rizik MeSH
- interleukin-10 krev MeSH
- interleukin-12 krev MeSH
- kombinovaná terapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- masti MeSH
- mladiství MeSH
- následné studie MeSH
- neparametrická statistika MeSH
- pravděpodobnost MeSH
- prediktivní hodnota testů MeSH
- psoriáza krev diagnóza terapie MeSH
- referenční hodnoty MeSH
- senioři MeSH
- senzitivita a specificita MeSH
- studie případů a kontrol MeSH
- stupeň závažnosti nemoci MeSH
- terapie ultrafialovými paprsky metody MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- biologické markery MeSH
- cytokiny MeSH
- dehet uhelný MeSH
- interleukin-10 MeSH
- interleukin-12 MeSH
- masti MeSH
Both CD8+ and CD4+ T cells with specific activity against tumor antigens are needed for an efficient antitumor immune response. Activation and proliferation of T cells require cellular interactions including adhesion, recognition of peptides presented by MHC molecules to the T cells receptor, and costimulation. In a series of experiments we attempted to generate and expand specific T cells by repeated stimulation using antigen-loaded autologous dendritic cells (DCs). DCs were obtained from peripheral blood mononuclear cells (PBMC) in the presence of IL-4 and GM-CSF. TNF-a was added to induce maturation. A conjugate of myeloma idiotypic protein with keyhole limpet hemocyanin was used as antigen. Nonadherent peripheral blood mononuclear cells were cultured in the presence of Il-2 and IL-7. Autologous DCs were added to the lymphocyte cultures on days 3, 10, and 17. The lymphocytes were stimulated by high concentration of IL-2 between days 21 and 27. Lymphocytes harvested on day 27 proliferated in response to antigen-loaded DC but failed to do so if less than 0.3 x 10(6) DCs were added for stimulation during culture. However, no cytotoxic activity against autologous DCs was detected and IFN-g production in the T cell cultures was low at the end of culture. In conclusion, the generation and expansion of T cells using repeated stimulation by autologous DCs is feasible but defective cytotoxic response of these cells occurs, possibly as a consequence of repeated frequent exposure to antigen.
- MeSH
- aktivace lymfocytů MeSH
- CD antigeny imunologie MeSH
- cytokiny imunologie MeSH
- dendritické buňky účinky léků imunologie MeSH
- ELISA MeSH
- interleukin-10 krev MeSH
- interleukin-12 krev MeSH
- interleukin-2 farmakologie MeSH
- kultivované buňky MeSH
- lidé MeSH
- referenční hodnoty MeSH
- T-lymfocyty imunologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- CD antigeny MeSH
- cytokiny MeSH
- interleukin-10 MeSH
- interleukin-12 MeSH
- interleukin-2 MeSH
