Simkania negevensis is an obligate intracellular bacterium grouped into the order Chlamydiales. This new amoeba-resistant bacterium represents a novel aetiologic agent of bronchiolitis and community-acquired pneumonia in both adults and children. It has been suggested that Simkania could be an ubiquitous microorganism presented in water environments. In the natural history of infections with amoeba-related bacteria encountered in aquatic habitats, the transmissions by environmental aerosols or contaminated water/air systems have been extensively recognized. Therefore, understanding the feasibility of Simkania infection by these or similar routes is relevant. In the present work, we investigated the prevalence of this novel disease-associated microorganism in water samples from different sources by real-time PCR (qPCR). Our results show Simkania detection in 5 of 185 water analyzed samples (2.7%: 2 of 88 cooling towers and 3 of 8 waste water samples). However, no Simkania was detected in a drinking water.

• MeSH
• Chlamydiales klasifikace   genetika   izolace a purifikace   MeSH
• infekce dýchací soustavy mikrobiologie   MeSH
• mikrobiologie vody * MeSH
• znečištění vody * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Španělsko MeSH

Chlamydia psittaci var. felis is considered as a primary and important agent in the etiology of infectious diseases of the upper respiratory tract and eyes in cats, having zoonotic potential. We investigated 13 cats aged between 2 months and 7 years, in which conjunctivitis, rhinitis, laryngotracheitis, bronchopneumonia and lymph adenopathy was clinically diagnosed. To detect the antigen of C. psittaci the Clearview Chlamydia Direct Test was used for the first time. The presence of C psittaci in 10 of 13 investigated cats and in 8 nasal mucosa smears from 10 investigated cats were confirmed in conjunctiva. Using bacteriological examination different species of the genus Staphylococcus in conjunctiva from all 13 investigated animals were also confirmed. Serological investigation using complement fixation test was negative in all animals.

• MeSH
• bakteriální konjunktivitida diagnóza   mikrobiologie   veterinární   MeSH
• chlamydiové infekce diagnóza   mikrobiologie   přenos   veterinární   MeSH
• Chlamydophila psittaci izolace a purifikace   MeSH
• imunologické testy veterinární   MeSH
• infekce dýchací soustavy diagnóza   mikrobiologie   veterinární   MeSH
• kočky MeSH
• lidé MeSH
• nemoci koček diagnóza   mikrobiologie   MeSH
• sérologické testy veterinární   MeSH
• stafylokokové infekce diagnóza   mikrobiologie   přenos   veterinární   MeSH
• zoonózy mikrobiologie   přenos   MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH