The effect of enhanced laccase (Lac) activity (obtained after copper addition to cultivation media) on decolorization of azo dye Orange G in two basidiospore-derived monokaryotic isolates of Pleurotus ostreatus was determined. The high Lac-producing isolate efficiently decolorized Orange G. The low-producing isolate showed only poor decolorization ability during cultivation in liquid medium and no decolorization on agar plates containing Orange G after a 25-d growth. A substantial enhancement of Lac activity caused by copper addition into cultivation media was detected in both isolates but, at the same time, the biomass production decreased and decolorization rate was reduced.

• MeSH
• Enzyme Activation drug effects   MeSH
• Azo Compounds metabolism   MeSH
• Laccase metabolism   MeSH
• Environmental Pollutants metabolism   MeSH
• Copper pharmacology   MeSH
• Peroxidases metabolism   MeSH
• Pleurotus classification   enzymology   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Azo Compounds MeSH
• Laccase MeSH
• Environmental Pollutants MeSH
• manganese peroxidase MeSH Browser
• Copper MeSH
• Orange G MeSH Browser
• Peroxidases MeSH

The ability to decolorize four synthetic dyes (Phenol Red, Evans Blue, Eosin Yellowish and Poly B411) in five Pleurotus ostreatus strains (a parental strain and four isolates derived from it) was determined. Two of the isolates had markedly higher and other two substantially lower production of ligninolytic enzymes and hydrogen peroxide than the parental strain. Like the parental strain, the higher-producing isolates were able to decolorize all the tested dyes, but not to a higher extent than the parental strain. In contrast, two lower-producing isolates exhibited slow decolorization, which was incomplete even at the end of cultivation. Evans Blue and Eosin Yellowish strongly suppressed the growth of the strains, while Phenol Red and Poly B411 induced none or only a very slight growth reduction.

• MeSH
• Color MeSH
• Coloring Agents metabolism   MeSH
• Laccase MeSH
• Oxidoreductases metabolism   MeSH
• Hydrogen Peroxide metabolism   MeSH
• Peroxidases metabolism   MeSH
• Pleurotus chemistry   enzymology   metabolism   MeSH
• Textile Industry MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Coloring Agents MeSH
• Laccase MeSH
• manganese peroxidase MeSH Browser
• Oxidoreductases MeSH
• Hydrogen Peroxide MeSH
• Peroxidases MeSH