We present the results of analysis of membrane phosphoproteomes from individual morphological stages of Streptomyces coelicolor that reflect developmentally dependent heterogeneity and phosphorylation of intrinsic and externally added purified Strepomyces aureofaciens EF-Tu. Fast growing nonpathogenic Mycobacterium smegmatis was used as a non-differentiating actinomycetes comparative model. Streptomycetes membrane fraction was found to contain protein kinase(s) catalyzing phosphorylation of both its own and an externally added EF-Tu, whereas Mycobacterium membrane fraction contains protein kinase phosphorylating only its own EF-Tu.

• MeSH
• Cell Membrane chemistry   enzymology   metabolism   MeSH
• Peptide Elongation Factor Tu isolation & purification   metabolism   MeSH
• Phosphorylation MeSH
• Mycobacterium smegmatis chemistry   enzymology   metabolism   MeSH
• Protein Processing, Post-Translational * MeSH
• Protein Kinases isolation & purification   metabolism   MeSH
• Streptomyces chemistry   enzymology   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Comparative Study MeSH
• Names of Substances
• Peptide Elongation Factor Tu MeSH
• Protein Kinases MeSH

In vitro phosphorylation of EF-Tu was shown in cell-free extract from dormant spores of Streptomyces coelicolor by a protein kinase present in spores. EF-Tu phosphorylation was observed on both intrinsic S. coelicolor factor and externally added purified EF-Tu from S. aureofaciens, on two isoforms. Putative serine and threonine residues as potential phosphorylation targets were determined in primary sequence and demonstrated on 3D structure model of EF-Tu.

• MeSH
• Electrophoresis, Gel, Two-Dimensional MeSH
• Peptide Elongation Factor Tu chemistry   isolation & purification   metabolism   MeSH
• Phosphorylation MeSH
• Models, Molecular MeSH
• Molecular Sequence Data MeSH
• Protein Kinases metabolism   MeSH
• Amino Acid Sequence MeSH
• Sequence Alignment MeSH
• Spores, Bacterial enzymology   metabolism   MeSH
• Streptomyces aureofaciens metabolism   MeSH
• Streptomyces coelicolor metabolism   MeSH
• Protein Structure, Tertiary MeSH
• Imaging, Three-Dimensional MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Peptide Elongation Factor Tu MeSH
• Protein Kinases MeSH