The effect of biotic stress induced by viral infection (Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants (Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVY(O). The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVY(NTN) strain than in the case of strain PVY(O). The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

• Klíčová slova
• NADP-malic enzyme, Nicotiana tabacum L., Potato virus Y strain NTN (PVYNTN), Potato virus Y strain O (PVYO), biotic stress, real time-PCR,
• MeSH
• kořeny rostlin metabolismus   virologie   MeSH
• listy rostlin metabolismus   virologie   MeSH
• malátdehydrogenasa chemie   genetika   metabolismus   MeSH
• messenger RNA metabolismus   MeSH
• nemoci rostlin virologie   MeSH
• Potyvirus patogenita   MeSH
• protein - isoformy chemie   genetika   metabolismus   MeSH
• stonky rostlin metabolismus   virologie   MeSH
• tabák enzymologie   virologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• malátdehydrogenasa MeSH
• malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) MeSH Prohlížeč
• messenger RNA MeSH
• protein - isoformy MeSH