Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-α m RNA expression in monocytes. We performed qPCR to determine relative telomere length (T/S ratios) in plasma, serum and whole blood of 36 volunteers. Using paired samples of plasma and serum and DNase treatment, we analysed the contribution of cfDNA to the co-activation of TNF-α mRNA expression in THP1 monocytic cell line. We found significant differences between paired plasma and serum samples in relative T/S ratios (median 1.38 ± 1.1 vs. 0.86 ± 0.25, respectively) and in total amounts of cfDNA and in estimated total amounts of telomeres which were significantly higher in serum than in plasma. TNF-α mRNA expression in THP1 cells increased significantly after DNase treatment of all samples used for stimulation. The highest TNF-α mRNA expressions were observed after stimulation with DNase treated serum samples. Our results suggest that the different content of telomeric sequences in plasma and serum may contribute to the tuning of immune response. Further studies of this interesting phenomenon are needed.

• MeSH
• deoxyribonukleasy metabolismus   MeSH
• homeostáza telomer MeSH
• imunita MeSH
• imunomodulace MeSH
• krevní plazma imunologie   metabolismus   MeSH
• lidé MeSH
• mladý dospělý MeSH
• monocyty fyziologie   MeSH
• sérum imunologie   metabolismus   MeSH
• telomery genetika   MeSH
• THP-1 buňky MeSH
• TNF-alfa genetika   metabolismus   MeSH
• upregulace MeSH
• volné cirkulující nukleové kyseliny genetika   imunologie   metabolismus   MeSH
• zdraví dobrovolníci pro lékařské studie MeSH
• Check Tag
• lidé MeSH
• mladý dospělý MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• deoxyribonukleasy MeSH
• TNF-alfa MeSH
• volné cirkulující nukleové kyseliny MeSH