Glucose-induced acid extrusion, respiration and anaerobic fermentation in baker's yeast was studied with the aid of sixteen inhibitors. Uranyl(2+) nitrate affected the acid extrusion more anaerobically than aerobically; the complexing of Mg2+ and Ca2+ by EDTA at the membrane had no effect. Inhibitors of glycolysis (iodoacetamide, N-ethylmaleimide, fluoride) suppressed acid production markedly, and so did the phosphorylation-blocking arsenate. Fluoroacetate, inhibiting the citric-acid cycle, had no effect. Inhibition by uncouplers depended on their pKa values: 2,4,6-trinitrophenol (pKa 0.4) less than 2,4-dinitrophenol (4.1) less than azide (4.7) less than 3-chlorophenylhydrazonomalononitrile (6.0). Inhibition by trinitrophenol was only slightly increased by its acetylation. Cyanide and nonpermeant oligomycin showed practically no effect; inhibition by dicyclohexylcarbodiimide was delayed but potent. The concentration profiles of inhibition of acid production differed from those of respiration and fermentation. Thus, though the acid production is a metabolically dependent process, it does not reflect the intensity of metabolism, except partly in the first half of glycolysis.

• MeSH
• arseničnany farmakologie   MeSH
• azidy farmakologie   MeSH
• dicyklohexylkarbodiimid farmakologie   MeSH
• dinitrofenoly farmakologie   MeSH
• dusičnan uranylu farmakologie   MeSH
• EDTA farmakologie   MeSH
• ethanol farmakologie   MeSH
• ethylmaleimid farmakologie   MeSH
• fluorid sodný farmakologie   MeSH
• jodacetamid farmakologie   MeSH
• kyanid draselný farmakologie   MeSH
• kyseliny metabolismus   MeSH
• nitrily farmakologie   MeSH
• oligomyciny farmakologie   MeSH
• pikráty farmakologie   MeSH
• Saccharomyces cerevisiae metabolismus   MeSH
• trinitrobenzeny farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• arseničnany MeSH
• azidy MeSH
• dicyklohexylkarbodiimid MeSH
• dinitrofenoly MeSH
• dusičnan uranylu MeSH
• EDTA MeSH
• ethanol MeSH
• ethylmaleimid MeSH
• fluorid sodný MeSH
• jodacetamid MeSH
• kyanid draselný MeSH
• kyseliny MeSH
• nitrily MeSH
• oligomyciny MeSH
• pikráty MeSH
• trinitrobenzeny MeSH

The effect of ten derivatives of carbonylcyanide phenylhydrazone on growth of bacteria, yeast and different species of filamentous fungi was investigated. In yeast and mitochondria isolated from rat liver the effect of these derivatives on the respiratory activity was also followed. The relative efficiency of the individual derivatives of carbonylcyanide phenylhydrazone was determined on the basis of the results obtained. It was shown that derivatives, in which the substituent on the benzene ring causes simultaneously an increase of acidity and lipophilicity of the derivative as compared with the non-substituted carbonylcyanide phenylhydrazone (4-trifluoromethoxy-, 3-chloro-, 4-chloro and 3,4-dichloro-derivatives) were most effective.

• MeSH
• Bacillus cereus růst a vývoj   MeSH
• houby růst a vývoj   MeSH
• jaterní mitochondrie metabolismus   MeSH
• karbonylkyanid-m-chlorfenylhydrazon analogy a deriváty   farmakologie   MeSH
• karbonylkyanid-p-trifluormethoxyfenylhydrazon farmakologie   MeSH
• krysa rodu Rattus MeSH
• nitrily farmakologie   MeSH
• Saccharomyces cerevisiae růst a vývoj   metabolismus   MeSH
• spotřeba kyslíku MeSH
• vztahy mezi strukturou a aktivitou MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• karbonylkyanid-m-chlorfenylhydrazon MeSH
• karbonylkyanid-p-trifluormethoxyfenylhydrazon MeSH
• nitrily MeSH

• MeSH
• cystein farmakologie   MeSH
• ethanol metabolismus   MeSH
• fenylhydraziny farmakologie   MeSH
• fermentace účinky léků   MeSH
• glukosa metabolismus   MeSH
• kvasinky účinky léků   metabolismus   MeSH
• kyanidy farmakologie   MeSH
• laktáty metabolismus   MeSH
• oxidativní fosforylace účinky léků   MeSH
• pyruváty metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cystein MeSH
• ethanol MeSH
• fenylhydraziny MeSH
• glukosa MeSH
• kyanidy MeSH
• laktáty MeSH
• pyruváty MeSH