Transmembrane adaptor proteins (TRAPs) are important organizers and regulators of immunoreceptor-mediated signaling. A bioinformatic search revealed several potential novel TRAPs, including a highly conserved protein, proline rich 7 (PRR7), previously described as a component of the PSD-95/N-methyl-d-aspartate receptor protein complex in postsynaptic densities (PSD) of rat neurons. Our data demonstrate that PRR7 is weakly expressed in other tissues but is readily up-regulated in activated human peripheral blood lymphocytes. Transient overexpression of PRR7 in Jurkat T cell line led to gradual apoptotic death dependent on the WW domain binding motif surrounding Tyr-166 in the intracellular part of PRR7. To circumvent the pro-apoptotic effect of PRR7, we generated Jurkat clones with inducible expression of PRR7 (J-iPRR7). In these cells acute induction of PRR7 expression had a dual effect. It resulted in up-regulation of the transcription factor c-Jun and the activation marker CD69 as well as enhanced production of IL-2 after phorbol 12-myristate 13-acetate (PMA) and ionomycin treatment. On the other hand, expression of PRR7 inhibited general tyrosine phosphorylation and calcium influx after T cell receptor cross-linking by antibodies. Moreover, we found PRR7 constitutively tyrosine-phosphorylated and associated with Src. Collectively, these data indicate that PRR7 is a potential regulator of signaling and apoptosis in activated T cells.

• MeSH
• adaptorové proteiny signální transdukční biosyntéza   genetika   imunologie   MeSH
• aminokyselinové motivy MeSH
• apoptóza účinky léků   fyziologie   MeSH
• Caco-2 buňky MeSH
• CD antigeny biosyntéza   genetika   imunologie   MeSH
• diferenciační antigeny T-lymfocytů biosyntéza   genetika   imunologie   MeSH
• fosforylace účinky léků   fyziologie   MeSH
• HEK293 buňky MeSH
• interleukin-2 biosyntéza   genetika   imunologie   MeSH
• ionofory farmakologie   MeSH
• ionomycin farmakologie   MeSH
• Jurkat buňky MeSH
• karcinogeny farmakologie   MeSH
• krysa rodu Rattus MeSH
• lektiny typu C biosyntéza   genetika   imunologie   MeSH
• lidé MeSH
• protoonkogenní proteiny c-jun genetika   imunologie   metabolismus   MeSH
• receptory antigenů T-buněk genetika   imunologie   metabolismus   MeSH
• regulace genové exprese fyziologie   MeSH
• T-lymfocyty imunologie   metabolismus   MeSH
• terciární struktura proteinů MeSH
• tetradekanoylforbolacetát farmakologie   MeSH
• U937 buňky MeSH
• vápníková signalizace účinky léků   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adaptorové proteiny signální transdukční MeSH
• CD antigeny MeSH
• CD69 antigen MeSH Prohlížeč
• diferenciační antigeny T-lymfocytů MeSH
• IL2 protein, human MeSH Prohlížeč
• interleukin-2 MeSH
• ionofory MeSH
• ionomycin MeSH
• karcinogeny MeSH
• lektiny typu C MeSH
• protoonkogenní proteiny c-jun MeSH
• receptory antigenů T-buněk MeSH
• tetradekanoylforbolacetát MeSH