A spontaneous mutant of Methanothermobacter thermautotrophicus resistant toward the ATP-synthase inhibitor N,N'-dicyclohexylcarbodiimide (DCCD) was isolated. DCCD normally inhibits methanogenic electron-transport-driven ATP synthesis, however, the DCCD-resistant strain exhibited methanogenesis in the presence of 300 micromol/L DCCD. Total ATP synthesis was shown to be higher in the mutant strain, both in the presence and absence of DCCD. These results suggested a modification in the ATP-synthesizing system of the mutant strain. Using Blue Native PAGE combined with MALDI TOF/TOF mass spectrometry, increased concentrations of both the A(1) and A(o) subcomplexes of the A(1)A(o)-type synthase were identified in the mutant strain. However, no alterations were found in the structural genes (atp) for the A(1)A(o) ATP synthase. The results imply that DCCD resistance is a consequence of increased A(1)A(o) ATP synthase expression, and suggest that genes involved in regulating synthase expression are responsible for DCCD resistance.

• MeSH
• adenosintrifosfát metabolismus   MeSH
• archeální proteiny biosyntéza   MeSH
• dicyklohexylkarbodiimid toxicita   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• exprese genu MeSH
• inhibitory enzymů toxicita   MeSH
• léková rezistence * MeSH
• methan metabolismus   MeSH
• Methanobacteriaceae chemie   účinky léků   izolace a purifikace   metabolismus   MeSH
• mutace * MeSH
• oxidace-redukce MeSH
• protonové ATPasy biosyntéza   MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
• upregulace MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adenosintrifosfát MeSH
• archeální proteiny MeSH
• dicyklohexylkarbodiimid MeSH
• inhibitory enzymů MeSH
• methan MeSH
• protonové ATPasy MeSH