The cell/tissue engineering therapy of extensive or chronic skin wounds is a highly topical task of the contemporary medicine. One of possible therapeutic approaches is grafting of in vitro cultured keratinocytes directly to the wound bed, where the cells colonize the wound, proliferate and improve the re-epithelization process. Because the successful cultivation of keratinocytes needs an application of feeder cells, the exclusion of these cells from the cultivation system is highly required. In this study we show a positive influence of 2-ethoxyethyl methacrylate as a component of cultivation support on growth of keratinocytes without feeder cells. Keratinocytes cultured on these surfaces are able to migrate to the model wound bed in vitro, where they form distinct colonies and have a normal differentiation potential.

• MeSH
• Biocompatible Materials chemistry   MeSH
• Cell Culture Techniques MeSH
• Keratinocytes cytology   physiology   MeSH
• Culture Media MeSH
• Cells, Cultured MeSH
• Humans MeSH
• Methacrylates chemistry   MeSH
• Polymers chemistry   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Biocompatible Materials MeSH
• Culture Media MeSH
• Methacrylates MeSH
• Polymers MeSH

Macroporous hydrogels based on 2-hydroxyethyl methacrylate, 2-ethoxyethyl methacrylate and N-(2-hydroxypropyl)methacrylamide, methacrylic acid and [2-(methacryloyloxy)ethyl]trimethylammonium chloride crosslinked with N,O-dimethacryloylhydroxylamine were prepared. Hydrogels were degraded in a buffer of pH 7.4. Completely water-soluble polymers were obtained over time periods ranging from 2 to 40 days. The process of degradation was followed gravimetrically and by optical and electron microscopy. In vivo biological tests with hydrogels based on copolymers of 2-ethoxyethyl methacrylate/N-(2-hydroxypropyl)methacrylamide were performed.

• MeSH
• Biocompatible Materials metabolism   therapeutic use   MeSH
• Hydrogels metabolism   therapeutic use   MeSH
• Hydrolysis MeSH
• Rats MeSH
• Methacrylates metabolism   therapeutic use   MeSH
• Spinal Cord Diseases pathology   therapy   MeSH
• Porosity MeSH
• Rats, Wistar MeSH
• Materials Testing methods   MeSH
• Absorbable Implants * MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Biocompatible Materials MeSH
• Hydrogels MeSH
• hydroxyethyl methacrylate MeSH Browser
• Methacrylates MeSH