Epithelial-mesenchymal interaction between stromal fibroblasts and cancer cells influences the functional properties of tumor epithelium, including the tumor progression and spread. We compared fibroblasts prepared from stroma of squamous cell carcinoma and normal dermal fibroblasts concerning their biological activity toward normal keratinocytes assessed by immunocytochemistry and profiling of gene activation for growth factors/cytokines by microarray chip technology. IGF-2 and BMP-4 were determined as candidate factors responsible for tumor-associated fibroblast activity that influences normal epithelia. This effect was confirmed by addition of recombinant IGF-2 and BMP4, respectively, to the culture medium. This hypothesis was also verified by inhibition experiments where blocking antibodies were employed in the medium conditioned by cancer-associated fibroblast. Presence of these growth factors was also detected in tumor samples.

• MeSH
• buňky NIH 3T3 MeSH
• čipová analýza proteinů MeSH
• fenotyp MeSH
• fibroblasty metabolismus   MeSH
• imunohistochemie MeSH
• insulinu podobný růstový faktor II biosyntéza   MeSH
• keratinocyty cytologie   metabolismus   MeSH
• kostní morfogenetický protein 4 biosyntéza   MeSH
• lidé MeSH
• myši MeSH
• nádory hlavy a krku metabolismus   patologie   MeSH
• rekombinantní proteiny biosyntéza   MeSH
• spinocelulární karcinom metabolismus   patologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• insulinu podobný růstový faktor II MeSH
• kostní morfogenetický protein 4 MeSH
• rekombinantní proteiny MeSH