Reduced beta cell mass in pancreatic islets (PI) of Goto-Kakizaki (GK) rats is frequently observed in this diabetic model, but knowledge on delta cells is scarce. Aiming to compare delta cell physiology/pathology of GK to Wistar rats, we found that delta cell number increased over time as did somatostatin mRNA and delta cells distribution in PI is different in GK rats. Subtle changes in 6-week-old GK rats were found. With maturation and aging of GK rats, disturbed cytoarchitecture occurred with irregular beta cells accompanied by delta cell hyperplasia and loss of pancreatic polypeptide (PPY) positivity. Unlike the constant glucose-stimulation index for insulin PI release in Wistar rats, this index declined with GK age, whereas for somatostatin it increased with age. A decrease of GK rat PPY serum levels was found. GK rat body weight decreased with increasing hyperglycemia. Somatostatin analog octreotide completely blocked insulin secretion, impaired proliferation at low autocrine insulin, and decreased PPY secretion and mitochondrial DNA in INS-1E cells. In conclusion, in GK rats PI, significant local delta cell hyperplasia and suspected paracrine effect of somatostatin diminish beta cell viability and contribute to the deterioration of beta cell mass. Altered PPY-secreting cells distribution amends another component of GK PI's pathophysiology.

• MeSH
• antagonisté inzulinu farmakologie   MeSH
• beta-buňky účinky léků   metabolismus   patologie   MeSH
• buňky vylučující somatostatin účinky léků   metabolismus   patologie   MeSH
• diabetes mellitus 2. typu krev   metabolismus   patologie   MeSH
• hyperplazie MeSH
• imunohistochemie MeSH
• inbrední kmeny potkanů MeSH
• inzulin metabolismus   MeSH
• inzulinová rezistence * MeSH
• messenger RNA metabolismus   MeSH
• nádorové buněčné linie MeSH
• oktreotid farmakologie   MeSH
• pankreatický polypeptid antagonisté a inhibitory   genetika   metabolismus   MeSH
• potkani Wistar MeSH
• proliferace buněk účinky léků   MeSH
• sekrece inzulinu MeSH
• somatostatin antagonisté a inhibitory   genetika   metabolismus   MeSH
• stárnutí * MeSH
• viabilita buněk účinky léků   MeSH
• vývojová regulace genové exprese MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antagonisté inzulinu MeSH
• inzulin MeSH
• messenger RNA MeSH
• oktreotid MeSH
• pankreatický polypeptid MeSH
• somatostatin MeSH