High hydrostatic pressure (HHP) has been shown to induce immunogenic cell death of cancer cells, facilitating their uptake by dendritic cells (DC) and subsequent presentation of tumor antigens. In the present study, we demonstrated immunogenicity of the HHP-treated tumor cells in mice. HHP was able to induce immunogenic cell death of both TC-1 and TRAMP-C2 tumor cells, representing murine models for human papilloma virus-associated tumors and prostate cancer, respectively. HHP-treated cells induced stronger immune responses in mice immunized with these tumor cells, documented by higher spleen cell cytotoxicity and increased IFNγ production as compared to irradiated tumor cells, accompanied by suppression of tumor growth in vivo in the case of TC-1 tumors, but not TRAMP-C2 tumors. Furthermore, HHP-treated cells were used for DC-based vaccine antigen pulsing. DC co-cultured with HHP-treated tumor cells and matured by a TLR 9 agonist exhibited higher cell surface expression of maturation markers and production of IL-12 and other cytokines, as compared to the DC pulsed with irradiated tumor cells. Immunization with DC cell-based vaccines pulsed with HHP-treated tumor cells induced high immune responses, detected by increased spleen cell cytotoxicity and elevated IFNγ production. The DC-based vaccine pulsed with HHP-treated tumor cells combined with docetaxel chemotherapy significantly inhibited growth of both TC-1 and TRAMP-C2 tumors. Our results indicate that DC-based vaccines pulsed with HHP-inactivated tumor cells can be a suitable tool for chemoimmunotherapy, particularly with regard to the findings that poorly immunogenic TRAMP-C2 tumors were susceptible to this treatment modality.

• MeSH
• Antigens, Neoplasm metabolism   MeSH
• Cytotoxicity, Immunologic MeSH
• Dendritic Cells cytology   MeSH
• Docetaxel MeSH
• Neoplasms, Experimental drug therapy   therapy   MeSH
• Hydrostatic Pressure MeSH
• Immune System MeSH
• Immunotherapy methods   MeSH
• Papillomavirus Infections drug therapy   therapy   MeSH
• Interferon-gamma metabolism   MeSH
• Interleukin-12 metabolism   MeSH
• Humans MeSH
• Mice, Inbred C57BL MeSH
• Mice MeSH
• Cell Line, Tumor MeSH
• Prostatic Neoplasms drug therapy   metabolism   therapy   MeSH
• Antineoplastic Agents administration & dosage   MeSH
• Cancer Vaccines chemistry   MeSH
• Spleen immunology   MeSH
• Taxoids administration & dosage   MeSH
• Toll-Like Receptor 9 metabolism   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Antigens, Neoplasm MeSH
• Docetaxel MeSH
• Interferon-gamma MeSH
• Interleukin-12 MeSH
• Antineoplastic Agents MeSH
• Cancer Vaccines MeSH
• Taxoids MeSH
• Tlr9 protein, mouse MeSH Browser
• Toll-Like Receptor 9 MeSH