The prominence of fentanyl and fentanyl analogues or Fentanyl Related Substances (FRS) has driven a nationwide crisis of opioid overdoses, which significantly presents an issue for public health and safety. Originally developed for medical purposes, fentanyl and FRS have become critical contributors to opioid overdose deaths due to their distribution, availability, and potency. This study examined toxicodynamic properties between butyrylcholinesterase (BChE) and fentanyl analogues via Ellman's assay. The enzymatic function of BChE was significantly inhibited by each of the 5 fentanyl analogues tested, which indicates the potential for utilization of this interaction. This reaction can be immobilized for a portable, single-use kit to detect FRS directly from any surface on-site. This would immensely benefit society by reducing the frequency of exposure and overdoses by providing additional safety measures to law enforcement and first responders.

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