A cosmid bearing an insert of 38 217 bp covering the gene cluster and its flanking regions of type strain Streptomyces lincolnensis ATCC 25466 was sequenced. Two relatively extensive sequence changes and several hundred point mutations were identified if compared with the previously published sequence of the lincomycin (Lin) industrial strain S. lincolnensis 78-11. Analysis of the cluster-flanking regions revealed its localization within the genome of the ATCC 25466 strain. The cluster-bearing cosmid was integrated into the chromosome of Lin non-producing strains S. coelicolor CH 999 and S. coelicolor M 145. The modified strains heterologously produced Lin but the level dropped to approximately 1-3% of the production in the ATCC 25466 strain.

• MeSH
• antibakteriální látky biosyntéza   chemie   MeSH
• bakteriální proteiny genetika   metabolismus   MeSH
• biotechnologie MeSH
• bodová mutace MeSH
• genová knihovna MeSH
• kosmidy MeSH
• linkomycin biosyntéza   chemie   MeSH
• multigenová rodina * MeSH
• sekvenční analýza DNA * MeSH
• Streptomyces coelicolor genetika   metabolismus   MeSH
• Streptomyces genetika   růst a vývoj   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antibakteriální látky MeSH
• bakteriální proteiny MeSH
• linkomycin MeSH