A cosmid bearing an insert of 38 217 bp covering the gene cluster and its flanking regions of type strain Streptomyces lincolnensis ATCC 25466 was sequenced. Two relatively extensive sequence changes and several hundred point mutations were identified if compared with the previously published sequence of the lincomycin (Lin) industrial strain S. lincolnensis 78-11. Analysis of the cluster-flanking regions revealed its localization within the genome of the ATCC 25466 strain. The cluster-bearing cosmid was integrated into the chromosome of Lin non-producing strains S. coelicolor CH 999 and S. coelicolor M 145. The modified strains heterologously produced Lin but the level dropped to approximately 1-3% of the production in the ATCC 25466 strain.

• MeSH
• Anti-Bacterial Agents biosynthesis   chemistry   MeSH
• Bacterial Proteins genetics   metabolism   MeSH
• Biotechnology MeSH
• Point Mutation MeSH
• Gene Library MeSH
• Cosmids MeSH
• Lincomycin biosynthesis   chemistry   MeSH
• Multigene Family * MeSH
• Sequence Analysis, DNA * MeSH
• Streptomyces coelicolor genetics   metabolism   MeSH
• Streptomyces genetics   growth & development   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Bacterial Proteins MeSH
• Lincomycin MeSH