Reactive oxygen species play an important role in the process of apoptosis in many cell types. In this paper, we analyzed the role of ROS in DNA-damaging agents (actinomycin D or decitabine), which induced apoptosis of leukemia cell line CML-T1 and normal peripheral blood lymphocytes (PBL). The possibility of synergism with histone deacetylase inhibitors butyrate or SAHA is also reported. We found that in cancer cell line, ROS production significantly contributed to apoptosis triggering, while in normal lymphocytes treated by cytostatic or cytotoxic drugs, necrosis as well as apoptosis occurred and large heterogeneity of ROS production was measured. Combined treatment with histone deacetylase inhibitor did not potentiate actinomycin D action, whereas combination of decitabine and SAHA brought synergistic ROS generation and apoptotic features in CML cell line. Appropriate decrease of cell viability indicated promising therapeutic potential of this combination in CML, but side effects on normal PBL should be taken into attention.

• MeSH
• Apoptosis drug effects   MeSH
• Azacitidine analogs & derivatives   pharmacology   MeSH
• Butyrates pharmacology   MeSH
• Cytostatic Agents pharmacology   MeSH
• Dactinomycin pharmacology   MeSH
• Decitabine MeSH
• Histone Deacetylase Inhibitors pharmacology   MeSH
• Caspase 3 metabolism   MeSH
• Hydroxamic Acids pharmacology   MeSH
• Humans MeSH
• Cell Line, Tumor MeSH
• Poly(ADP-ribose) Polymerases metabolism   MeSH
• DNA Damage drug effects   MeSH
• Antineoplastic Agents pharmacology   MeSH
• Reactive Oxygen Species metabolism   MeSH
• Drug Synergism MeSH
• Vorinostat MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Azacitidine MeSH
• Butyrates MeSH
• Cytostatic Agents MeSH
• Dactinomycin MeSH
• Decitabine MeSH
• Histone Deacetylase Inhibitors MeSH
• Caspase 3 MeSH
• Hydroxamic Acids MeSH
• Poly(ADP-ribose) Polymerases MeSH
• Antineoplastic Agents MeSH
• Reactive Oxygen Species MeSH
• Vorinostat MeSH