Group A Rotaviruses (RVA) are the leading cause of acute gastroenteritis in children and a major cause of childhood mortality in low-income countries. RVAs are mostly host-specific, but interspecies transmission and reassortment between human and animal RVAs significantly contribute to their genetic diversity. We investigated the VP7 and VP4 genotypes of RVA isolated from 225 stool specimens collected from Czech patients with gastroenteritis during 2016-2019. The most abundant genotypes were G1P[8] (42.7%), G3P[8] (11.1%), G9P[8] (9.8%), G2P[4] (4.4%), G4P[8] (1.3%), G12P[8] (1.3%), and, surprisingly, G8P[8] (9.3%). Sequence analysis of G8P[8] strains revealed the highest nucleotide similarity of all Czech G8 sequences to the G8P[8] rotavirus strains that were isolated in Vietnam in 2014/2015. The whole-genome backbone of the Czech G8 strains was determined with the use of next-generation sequencing as DS-1-like. Phylogenetic analysis of all segments clustered the Czech isolates with RVA strains that were formerly described in Southeast Asia, which had emerged following genetic reassortment between bovine and human RVAs. This is the first time that bovine-human DS1-like G8P[8] strains were detected at a high rate in human patients in Central Europe. Whether the emergence of this unusual genotype reflects the establishment of a new RVA strain in the population requires the continuous monitoring of rotavirus epidemiology.

• Klíčová slova
• Central Europe, G8, bovine–human reassortants, gastroenteritis, rotavirus A,
• MeSH
• antigeny virové genetika   MeSH
• feces virologie   MeSH
• fylogeneze MeSH
• gastroenteritida epidemiologie   virologie   MeSH
• genom virový genetika   MeSH
• genotyp MeSH
• lidé MeSH
• prevalence MeSH
• reassortantní viry genetika   izolace a purifikace   MeSH
• RNA virová genetika   MeSH
• rotavirové infekce epidemiologie   virologie   MeSH
• Rotavirus genetika   izolace a purifikace   MeSH
• skot MeSH
• virové plášťové proteiny genetika   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• multicentrická studie MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH
• Názvy látek
• antigeny virové MeSH
• RNA virová MeSH
• virové plášťové proteiny MeSH
• VP4 protein, Rotavirus MeSH Prohlížeč
• VP7 protein, Rotavirus MeSH Prohlížeč