• Klíčová slova
• Burkholderia, FT-IR, cystic fibrosis, epidemiology,
• MeSH
• Burkholderia cepacia komplex * MeSH
• Burkholderia * MeSH
• cystická fibróza * komplikace   MeSH
• infekce bakteriemi rodu Burkholderia * MeSH
• lidé MeSH
• spektroskopie infračervená s Fourierovou transformací metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• dopisy MeSH

Cystic fibrosis (CF) patients in the Czech Republic suffered in the late 1990s from an epidemic with ST32 strain of Burkholderia cepacia complex (Bcc). Cohort segregation of Bcc and of ST32 positive patients was introduced in 1999 and 2002, respectively. We performed a study to evaluate the molecular epidemiology of Bcc infection after implementation of these infection control measures. Patients attending the Prague CF adult Centre from 2000 to 2015 were included in the present study. Demographic data and microbial statuses were collected from patient records. All Bcc isolates were analyzed using multilocus sequence typing (MLST). The prevalences of epidemic strain ST32 and of other Bcc strains were calculated. Ninety out of 227 CF patients were infected with Bcc during the study period. The prevalence of ST32 cases significantly decreased from 46.5% in 2000-2001 to 10.4% in 2014-2015 (P < 0.001) due to occurrence of only one new case in 2003, as well as to the death of 72% of ST32-infected patients. Conversely, there was a significant increase in prevalence of other Bcc strains, which rose from 0 to 14.9% (P = 0.015) and of transient infections. A micro-epidemic of infection with ST630 strain was observed in 2014 in lung transplant patients hospitalized in intensive care unit. The prevalence of epidemic strain ST32 decreased, whereas that of non-clonal strains of Bcc increased. Routine use of MLST allowed early detection of new and potentially epidemic strains.

• Klíčová slova
• Cystic Fibrosis, Cystic Fibrosis Patient, Molecular Genetic Method, Multilocus Sequence Typing, recA Group,
• MeSH
• Burkholderia cepacia komplex klasifikace   genetika   izolace a purifikace   MeSH
• cystická fibróza epidemiologie   mikrobiologie   MeSH
• infekce bakteriemi rodu Burkholderia epidemiologie   mikrobiologie   MeSH
• lidé MeSH
• molekulární epidemiologie MeSH
• multilokusová sekvenční typizace MeSH
• prevalence MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH

The association between oxidative stress and neutrophilic inflammation in cystic fibrosis (CF) lung disease is well recognized. 8-Isoprostane is a product of non-enzymatic oxidation of arachidonic acid. The aim of the present study was to examine the relationship between lung function decline and 8-isoprostane concentrations in exhaled breath condensate (EBC) in CF patients with Burkholderia cenocepacia airway colonization. Concentrations of 8-isoprostane in EBC were measured in 24 stable CF patients with B. cenocepacia airway colonization. The median (interquartile range) age of the cohort was 23.9 (22.0; 26.6) years. All patients underwent clinical examinations and pulmonary function tests at the time of EBC collection and in 1-, 3-, and 5-year intervals. 8-Isoprostane concentrations in EBC correlated to 1- and 3-year declines of forced expiratory volume in 1 s (FEV1) with r(S) values of -0.511 (p = 0.0011) and -0.495 (p = 0.016), respectively. In multiple regression analysis, 8-isoprostane concentrations in EBC were the only independent predictor for 1-year FEV1 decline (p = 0.01). When the median value of 8-isoprostane concentration in EBC (10.0 pg/mL) was used as a cutoff, subgroups of patients with lower and higher level of oxidative stress had significantly different median (interquartile range) FEV1 declines in 1-year interval, -2.4% (-5.3; 0.8) and -7.3% (-10.3; -5.8) predicted (p = 0.009). In conclusion, 8-isoprostane concentrations in EBC correlated to short-term lung function decline in CF patients with B. cenocepacia airway colonization. This correlation reflects the role of oxidative stress in CF lung pathogenesis and contributes to prediction of prognosis in these patients.

• MeSH
• Burkholderia cenocepacia * MeSH
• časové faktory MeSH
• cystická fibróza komplikace   metabolismus   patofyziologie   MeSH
• dinoprost analogy a deriváty   metabolismus   MeSH
• dospělí MeSH
• infekce bakteriemi rodu Burkholderia komplikace   mikrobiologie   MeSH
• lidé MeSH
• mladý dospělý MeSH
• oportunní infekce * MeSH
• oxidační stres * MeSH
• respirační funkční testy MeSH
• usilovný výdechový objem MeSH
• vydechnutí MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 8-epi-prostaglandin F2alpha MeSH Prohlížeč
• dinoprost MeSH