The freshwaters of Iraq harbour a high diversity of endemic and phylogenetically unique species. One of the most diversified fish groups in this region is cyprinoids, and although their distribution is relatively well known, their monogenean parasites have only rarely been investigated. Herein, we applied an integrative approach, combining morphology with molecular data, to assess the diversity and phylogeny of cyprinoid-associated monogenean parasites. A total of 33 monogenean species were collected and identified from 13 endemic cyprinoid species. The highest species diversity was recorded for Dactylogyrus (Dactylogyridae, 16 species) and Gyrodactylus (Gyrodactylidae, 12 species). Four species of Dactylogyrus and 12 species of Gyrodactylus were identified as new to science and described. Two other genera, Dogielius (Dactylogyridae) and Paradiplozoon (Diplozoidae), were represented only by 4 and 1 species, respectively. Phylogenetic analyses of the Dactylogyrus and Gyrodactylus species revealed that the local congeners do not form a monophyletic group and are phylogenetically closely related to species from other regions (i.e. Europe, North Africa and Eastern Asia). These findings support the assumption that the Middle East served as an important historical crossroads for the interchange of fauna between these 3 geographic regions.

• Keywords
• Cyprinoidei, Dactylogyrus, Dogielius, Gyrodactylus, Middle East, Paradiplozoon, phylogeny, species diversity,
• MeSH
• Phylogeny MeSH
• Fishes MeSH
• Trematoda * genetics   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Iraq epidemiology   MeSH
• Africa, Northern MeSH
• Middle East MeSH

BACKGROUND: Monogenean flatworms are the main ectoparasites of fishes. Representatives of the species-rich families Gyrodactylidae and Dactylogyridae, especially those infecting cichlid fishes and clariid catfishes, are important parasites in African aquaculture, even more so due to the massive anthropogenic translocation of their hosts worldwide. Several questions on their evolution, such as the phylogenetic position of Macrogyrodactylus and the highly speciose Gyrodactylus, remain unresolved with available molecular markers. Also, diagnostics and population-level research would benefit from the development of higher-resolution genetic markers. We aim to offer genetic resources for work on African monogeneans by providing mitogenomic data of four species (two belonging to Gyrodactylidae, two to Dactylogyridae), and analysing their gene sequences and gene order from a phylogenetic perspective. RESULTS: Using Illumina technology, the first four mitochondrial genomes of African monogeneans were assembled and annotated for the cichlid parasites Gyrodactylus nyanzae, Cichlidogyrus halli, Cichlidogyrus mbirizei (near-complete mitogenome) and the catfish parasite Macrogyrodactylus karibae (near-complete mitogenome). Complete nuclear ribosomal operons were also retrieved, as molecular vouchers. The start codon TTG is new for Gyrodactylus and for Dactylogyridae, as is the incomplete stop codon TA for Dactylogyridae. Especially the nad2 gene is promising for primer development. Gene order was identical for protein-coding genes and differed between the African representatives of these families only in a tRNA gene transposition. A mitochondrial phylogeny based on an alignment of nearly 12,500 bp including 12 protein-coding and two ribosomal RNA genes confirms that the Neotropical oviparous Aglaiogyrodactylus forficulatus takes a sister group position with respect to the other gyrodactylids, instead of the supposedly 'primitive' African Macrogyrodactylus. Inclusion of the African Gyrodactylus nyanzae confirms the paraphyly of Gyrodactylus. The position of the African dactylogyrid Cichlidogyrus is unresolved, although gene order suggests it is closely related to marine ancyrocephalines. CONCLUSIONS: The amount of mitogenomic data available for gyrodactylids and dactylogyrids is increased by roughly one-third. Our study underscores the potential of mitochondrial genes and gene order in flatworm phylogenetics, and of next-generation sequencing for marker development for these non-model helminths for which few primers are available.

• Keywords
• Cichlidae, Cichlidogyrus, Clariidae, Gene order, Gyrodactylus, Macrogyrodactylus, Mitogenome, Monogenea, Monopisthocotylea, Phylogenomics,
• MeSH
• Cichlids parasitology   MeSH
• Phylogeny MeSH
• Genome, Mitochondrial MeSH
• Mitochondria classification   genetics   MeSH
• Platyhelminths genetics   MeSH
• Gene Order MeSH
• DNA, Protozoan chemistry   isolation & purification   metabolism   MeSH
• Protozoan Proteins classification   genetics   MeSH
• RNA, Ribosomal classification   genetics   MeSH
• Sequence Analysis, DNA MeSH
• High-Throughput Nucleotide Sequencing MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• DNA, Protozoan MeSH
• Protozoan Proteins MeSH
• RNA, Ribosomal MeSH

A new genus and species of monogenean belonging to the Gyrodactylidae, Citharodactylus gagei n. gen. et n. sp. (Plathyhelminthes, Monogenea), is described from the gills of the moon fish, Citharinus citharus (Geoffroy Saint-Hilaire), a characiform fish collected from Lake Turkana in northern Kenya. The new viviparous genus can be readily distinguished from the six other gyrodactylid genera recorded from Africa and from the other viviparous genera within the Gyrodactylidae based on the morphology of the male copulatory organ (MCO), which consists of a muscular ovate organ with an opening onto the tegument through which the narrow tapered end of a sclerotised curved cone-shaped structure protrudes. The tegumental opening of the MCO is surrounded by a collar of short spines. Sequencing of the nuclear ribosomal DNA internal transcribed spacers 1 and 2, the 5.8S and the 18S rDNA genes and a comparison with the gyrodactylid species listed in GenBank confirmed the specimens are unique and do not match with any existing entry. When phylogenies for each genomic region were conducted (i.e. 0.064 gamma-corrected pairwise genetic distance based on a alignment of 1750 bp of the 1857 bp long 18S rDNA gene), the most similar match was that of Afrogyrodactylus sp. [= A. girgifae (Folia Parasitol 61:529-536, 2014)] from Brycinus nurse (Rüppell). The proposed name of the new parasite is Citharodactylus n. gen. which represents the seventh gyrodactylid genus to be found in Africa and the 25th viviparous genus and the 32nd genus to be added to the Gyrodactylidae.

• Keywords
• Africa, Ectoparasite, New genus, New species, Parasite, Viviparous,
• MeSH
• Characiformes parasitology   MeSH
• Phylogeny MeSH
• Trematode Infections epidemiology   parasitology   veterinary   MeSH
• Lakes parasitology   MeSH
• DNA, Ribosomal Spacer genetics   MeSH
• Fish Diseases parasitology   MeSH
• Trematoda anatomy & histology   classification   genetics   isolation & purification   MeSH
• Gills parasitology   MeSH
• Animals MeSH
• Check Tag
• Male MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Kenya MeSH
• Names of Substances
• DNA, Ribosomal Spacer MeSH

New findings on Gyrodactylus spp. parasitising African cichlids in southern Africa are presented, comprising data from Zimbabwe and South Africa. Morphometry of opisthaptoral hard parts in combination with nuclear ribosomal DNA sequences confirmed the presence of six species of Gyrodactylus von Nordmann, 1832. Three new species are described from fishes in Zimbabwe: Gyrodactylus chitandiri n. sp. from the gill arches of Coptodon rendalli (Boulenger) and Pseudocrenilabrus philander (Weber); Gyrodactylus occupatus n. sp. from the fins of Oreochromis niloticus (L.), Pharyngochromis acuticeps (Steindachner) and P. philander; and Gyrodactylus parisellei n. sp. from the fins of O. niloticus, P. philander and Tilapia sp. Gyrodactylus nyanzae Paperna, 1973 was also identified from the gills of O. niloticus and C. rendalli collected from two localities in Zimbabwe; these findings represent new host and locality records for this parasite. Gyrodactylus sturmbaueri Vanhove, Snoeks, Volckaert & Huyse, 2011 was identified from P. philander collected in South Africa and Zimbabwe thereby providing new host and locality records for this parasite. Finally, Gyrodactylus yacatli García-Vásquez, Hansen, Christison, Bron & Shinn, 2011 was collected from the fins of O. niloticus and P. philander studied in Zimbabwe; this represents the first record of this species from the continent of Africa. Notably, this study improves upon the knowledge of Gyrodactylus spp. parasitising cichlids from these southern African regions. All species studied were recorded from at least two different cichlid host species indicating trend for a wide range of Gyrodactylus hosts in Africa. Accordingly, this supports the idea of intensive host switching in the course of their evolution.

• MeSH
• Biodiversity MeSH
• Cichlids parasitology   MeSH
• DNA, Helminth genetics   MeSH
• Species Specificity MeSH
• Phylogeny MeSH
• Host Specificity MeSH
• Animal Fins parasitology   MeSH
• Rivers MeSH
• DNA, Ribosomal genetics   MeSH
• Trematoda anatomy & histology   classification   genetics   MeSH
• Gills parasitology   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• South Africa MeSH
• Zimbabwe MeSH
• Names of Substances
• DNA, Helminth MeSH
• DNA, Ribosomal MeSH

Some taxonomic groups are less amenable to mitochondrial DNA barcoding than others. Due to the paucity of molecular information of understudied groups and the huge molecular diversity within flatworms, primer design has been hampered. Indeed, all attempts to develop universal flatworm-specific COI markers have failed so far. We demonstrate how high molecular variability and contamination problems limit the possibilities for barcoding using standard COI-based protocols in flatworms. As a consequence, molecular identification methods often rely on other widely applicable markers. In the case of Monogenea, a very diverse group of platyhelminth parasites, and Rhabdocoela, representing one-fourth of all free-living flatworm taxa, this has led to a relatively high availability of nuclear ITS and 18S/28S rDNA sequences on GenBank. In a comparison of the effectiveness in species assignment we conclude that mitochondrial and nuclear ribosomal markers perform equally well. In case intraspecific information is needed, rDNA sequences can guide the selection of the appropriate (i.e. taxon-specific) COI primers if available.

• Keywords
• Monogenea, Rhabdocoela, mitochondrial DNA, primer design, ribosomal DNA, turbellarians,
• Publication type
• Journal Article MeSH