Early and late thrombosis remain the most frequent reasons for the failure of synthetic cardiovascular grafts. Long-term hemocompatibility of implanted synthetic grafts can be achieved if a natural living endothelium is formed over its blood-contacting surface. Here we present a modification of a standard expanded polytetrafluorethylene (ePTFE) vessel prosthesis by a controlled preparation of a fibrin mesh enriched with covalently bound heparin and noncovalently bound vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF). Compared to a bare prosthesis, the coated prosthesis showed excellent antithrombogenic properties after contact with heparinized fresh human blood. Human umbilical vein endothelial cells seeded on the inner surface of the coated prosthesis formed a confluent layer in 5 days, whereas only small colonies of cells were scattered on the bare prosthesis. Viability of the cells was promoted mainly by FGF immobilized on the coating. These findings suggest that the coating may prevent acute thrombus formation and support the self-endothelialization of an implanted ePTFE vascular graft in vivo.

• Publication type
• Journal Article MeSH

The study monitored in vitro early response of connective tissue cells and immunocompetent cells to enosseal implant materials coated by different blood components (serum, activated plasma, and plasma/platelets) to evaluate human osteoblast proliferation and synthetic activity and inflammatory response presented as a cytokine profile of peripheral blood mononuclear cells (PBMCs) under conditions imitating the situation upon implantation. The cells were cultivated on coated Ti-plasma-sprayed (Ti-PS), Ti-etched (Ti-Etch), Ti-hydroxyapatite (Ti-HA), and ZrO2 surfaces. The plasma/platelets coating supported osteoblast proliferation only on osteoconductive Ti-HA and Ti-Etch whereas activated plasma enhanced proliferation on all surfaces. Differentiation (BAP) and IL-8 production remained unchanged or decreased irrespective of the coating and surface; only the serum and plasma/platelets-coated ZrO2 exhibited higher BAP and IL-8 expression. RANKL production increased on serum and activated plasma coatings. PBMCs produced especially cytokines playing role in inflammatory phase of wound healing, that is, IL-6, GRO-α, GRO, ENA-78, IL-8, GM-CSF, EGF, and MCP-1. Cytokine profiles were comparable for all tested surfaces; only ENA-78, IL-8, GM-CSF, and MCP-1 expression depended on materials and coatings. The activated plasma coating led to uniformed surfaces and represented a favorable treatment especially for bioinert Ti-PS and ZrO2 whereas all coatings had no distinctive effect on bioactive Ti-HA and Ti-Etch.

• MeSH
• Coated Materials, Biocompatible adverse effects   chemistry   MeSH
• Cell Line MeSH
• Chemokine CCL2 metabolism   MeSH
• Chemokine CXCL1 metabolism   MeSH
• Chemokine CXCL5 metabolism   MeSH
• Cytokines metabolism   MeSH
• Epidermal Growth Factor metabolism   MeSH
• Granulocyte-Macrophage Colony-Stimulating Factor metabolism   MeSH
• Wound Healing drug effects   MeSH
• Interleukin-6 metabolism   MeSH
• Interleukin-8 metabolism   MeSH
• Leukocytes, Mononuclear drug effects   metabolism   MeSH
• Humans MeSH
• Osteoblasts drug effects   metabolism   MeSH
• Cell Proliferation drug effects   MeSH
• Titanium adverse effects   chemistry   MeSH
• Inflammation metabolism   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Coated Materials, Biocompatible MeSH
• Chemokine CCL2 MeSH
• Chemokine CXCL1 MeSH
• Chemokine CXCL5 MeSH
• CXCL1 protein, human MeSH Browser
• CXCL5 protein, human MeSH Browser
• Cytokines MeSH
• Epidermal Growth Factor MeSH
• Granulocyte-Macrophage Colony-Stimulating Factor MeSH
• Interleukin-6 MeSH
• Interleukin-8 MeSH
• Titanium MeSH