Genetic stability of amylase activity after serial subcultivation experiments with amylolytic ruminal Streptococcus bovis strains was investigated. Two strains Amy+ and Amy- were obtained. Loss of amylase activity connected with the loss of plasmid DNA was not found in these strains. The presence of the gene responsible for the amylase activity in the chromosome of these strains was revealed by hybridization of the alpha-amylase gene on pJK108 against chromosomal DNA of S. bovis and Bacillus subtilis after a complete restriction with EcoRI.

• MeSH
• alfa-amylasy genetika   metabolismus   MeSH
• bachor mikrobiologie   MeSH
• Bacillus subtilis enzymologie   genetika   MeSH
• bakteriální geny * MeSH
• DNA bakterií genetika   MeSH
• DNA sondy MeSH
• plazmidy genetika   MeSH
• stabilita enzymů genetika   MeSH
• Streptococcus bovis enzymologie   genetika   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alfa-amylasy MeSH
• DNA bakterií MeSH
• DNA sondy MeSH

In 11% of tested Streptococcus bovis strains a conjugative transfer of tetracycline resistance was observed when mating experiments were carried out on membrane filters. The recipient strain used was S. bovis BM114 with chromosomal resistance to rifampicin. In addition, in two strains tetracycline resistance was transferred also to recipient strain Enterococcus faecium AL6. The transfer frequencies were in the range of 10(-6) to 10(-3). The donor strains were screened for the presence of plasmids and one up to four bands of plasmid DNA in all tested strains were revealed. In spite of that isolation of plasmid DNA was successful only in 53/4/114 transconjugants. Transconjugant 32/114 contained amylase activity which was higher than in the donor strain.

• MeSH
• bachor mikrobiologie   MeSH
• Enterococcus faecium genetika   MeSH
• konjugace genetická * MeSH
• R-plasmidy genetika   MeSH
• rezistence na tetracyklin genetika   MeSH
• Streptococcus bovis účinky léků   genetika   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH