Recently, an increasing incidence of HPV-induced oropharyngeal squamous cell carcinoma (OPSCC) has been observed. Moreover, locoregionally advanced stages require a combined modal approach, and the prognosis is poor. Therefore, it is essential to find early diagnostic and prognostic biomarkers. DNA methylation changes play a crucial role in the process of carcinogenesis and are often investigated as promising biomarkers in many types of cancer. For analysis of DNA methylation levels of selected tumour suppressor genes in HPV-positive and HPV-negative samples (including primary tumours and corresponding metastases of metastasizing OPSCCs, primary tumours of non-metastasizing OPSCCs, and control samples), methylation-specific MLPA and methylation-specific high-resolution melting analyses were used. A significant difference in methylation between OPSCCs and the control group was observed in WT1, PAX6 (P < 0.01) and CADM1, RARβ (P < 0.05) genes. CADM1 and WT1 hypermethylation was detected mostly in HPV-positive samples; all but one HPV-negative samples were unmethylated. Moreover, hypermethylation of PAX5 gene was observed in metastases compared with control samples and was also associated with shorter overall survival of all patients (P < 0.05). Associations described herein between promoter methylation of selected genes and clinicopathological data could benefit OPSCC patients in the future by improvement in screening, early detection, and prognosis of the disease.

• Klíčová slova
• Biomarker, DNA methylation, epigenetics, head and neck cancer, human papillomavirus, oropharyngeal cancer,
• MeSH
• aktivátorový protein specifický pro B-buňky genetika   MeSH
• Alphapapillomavirus * MeSH
• buněčná adhezní molekula 1 genetika   metabolismus   MeSH
• dlaždicobuněčné karcinomy hlavy a krku genetika   MeSH
• DNA metabolismus   MeSH
• infekce papilomavirem * komplikace   MeSH
• lidé MeSH
• metylace DNA MeSH
• nádory hlavy a krku * genetika   MeSH
• nádory orofaryngu * patologie   MeSH
• Papillomaviridae MeSH
• prognóza MeSH
• proteiny WT1 genetika   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aktivátorový protein specifický pro B-buňky MeSH
• buněčná adhezní molekula 1 MeSH
• CADM1 protein, human MeSH Prohlížeč
• DNA MeSH
• PAX5 protein, human MeSH Prohlížeč
• PAX6 protein, human MeSH Prohlížeč
• proteiny WT1 MeSH
• retinoic acid receptor beta MeSH Prohlížeč
• WT1 protein, human MeSH Prohlížeč