Most cited article - PubMed ID 2636261
Cloning of a thermostable alpha-amylase gene from Thermomonospora curvata and its expression in Streptomyces lividans
The gene pkwA coding for a typical WD-repeat protein was found in the chromosome of the bacterium Thermomonospora curvata CCM 3352. Until now WD-repeat proteins were through to be confined to eukaryotes.
- MeSH
- Actinomycetales enzymology genetics MeSH
- Genes, Bacterial MeSH
- Bacterial Proteins genetics MeSH
- Molecular Sequence Data MeSH
- Protein Serine-Threonine Kinases genetics MeSH
- Repetitive Sequences, Nucleic Acid * MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Homology, Amino Acid MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Bacterial Proteins MeSH
- PkwA protein, Thermomonospora curvata MeSH Browser
- Protein Serine-Threonine Kinases MeSH
DNA-Dependent RNA polymerase (EC 2.7.7.6) was isolated from Thermomonospora curvata. The purification steps included precipitation with Polymin P, elution of the precipitate with 0.3 mol/L KCl, precipitation with ammonium sulfate, affinity chromatography on heparin-agarose and molecular filtration on Biogel A 1.5 m.
- MeSH
- Actinomycetales enzymology MeSH
- Bacterial Proteins isolation & purification MeSH
- Chemical Precipitation MeSH
- Chromatography, Affinity MeSH
- DNA-Directed RNA Polymerases isolation & purification MeSH
- Chromatography, Gel MeSH
- Molecular Weight MeSH
- Spores, Bacterial MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Bacterial Proteins MeSH
- DNA-Directed RNA Polymerases MeSH