To investigate the effect of vanadyl trehalose (VT) on oxidative stress and reduced glutathione/glutathione-S-transferase (GSH/GSTs) pathway gene expression in mouse gastrointestinal tract, as well as the protective effects of vitamin C (VC) and reduced glutathione (GSH). Thirty male Kunming mice were randomly divided into five groups: control group (group A), VT group (group B), VC + VT group (group C), GSH + VT group (group D) and VC + GSH + VT group (group E). The content of reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) activity and the expressions of glutamate-cysteine ligase catalytic subunit (GCLC), glutathione synthetase (GSS), regulated through glutathione reductase (GSR) and glutathione-S-transferase pi (GSTpi) in stomach and duodenum in vanadyl trehalose treated group were lower than those in group A (P<0.05). The C, D, E group can significantly improve the above indicators, but those only in the stomach in E group reached the level of the control group. Vanadyl trehalose (VT) was able to cause oxidative stress damage to the gastrointestinal tract of mice, which affects GSH content and GSH-Px activity and interferes with the normal expression of GSH/GSTs pathway. Exogenous vitamin C, reduced glutathione and the combination of the two could play a specific role in antioxidant protection and reduce the toxicity of vanadyl trehalose.

• MeSH
• Antioxidants pharmacology   MeSH
• Biomimetics MeSH
• Glutathione metabolism   MeSH
• Glutathione Peroxidase metabolism   MeSH
• Glutathione Reductase metabolism   MeSH
• Glutathione Transferase metabolism   MeSH
• Hypoglycemic Agents pharmacology   MeSH
• Insulin pharmacology   MeSH
• Ascorbic Acid pharmacology   MeSH
• Mice MeSH
• Oxidative Stress MeSH
• Trehalose pharmacology   MeSH
• Vanadates pharmacology   MeSH
• Stomach drug effects   MeSH
• Animals MeSH
• Check Tag
• Male MeSH
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Antioxidants MeSH
• Glutathione MeSH
• Glutathione Peroxidase MeSH
• Glutathione Reductase MeSH
• Glutathione Transferase MeSH
• Hypoglycemic Agents MeSH
• Insulin MeSH
• Ascorbic Acid MeSH
• Trehalose MeSH
• Vanadates MeSH