GpsB regulatory protein and StkP protein kinase have been proposed as molecular switches that balance septal and peripheral (side-wall like) peptidoglycan (PG) synthesis in Streptococcus pneumoniae (pneumococcus); yet, mechanisms of this switching remain unknown. We report that ΔdivIVA mutations are not epistatic to ΔgpsB division-protein mutations in progenitor D39 and related genetic backgrounds; nor is GpsB required for StkP localization or FDAA labeling at septal division rings. However, we confirm that reduction of GpsB amount leads to decreased protein phosphorylation by StkP and report that the essentiality of ΔgpsB mutations is suppressed by inactivation of PhpP protein phosphatase, which concomitantly restores protein phosphorylation levels. ΔgpsB mutations are also suppressed by other classes of mutations, including one that eliminates protein phosphorylation and may alter division. Moreover, ΔgpsB mutations are synthetically lethal with Δpbp1a, but not Δpbp2a or Δpbp1b mutations, suggesting GpsB activation of PBP2a activity. Consistent with this result, co-IP experiments showed that GpsB complexes with EzrA, StkP, PBP2a, PBP2b and MreC in pneumococcal cells. Furthermore, depletion of GpsB prevents PBP2x migration to septal centers. These results support a model in which GpsB negatively regulates peripheral PG synthesis by PBP2b and positively regulates septal ring closure through its interactions with StkP-PBP2x.

• MeSH
• aminoacyltransferasy genetika   metabolismus   MeSH
• bakteriální proteiny genetika   metabolismus   MeSH
• buněčná stěna metabolismus   MeSH
• buněčné dělení genetika   fyziologie   MeSH
• faktory virulence genetika   metabolismus   MeSH
• fosforylace MeSH
• membránové proteiny genetika   metabolismus   MeSH
• mutace genetika   MeSH
• peptidoglykan biosyntéza   MeSH
• proteiny vázající penicilin genetika   metabolismus   MeSH
• Streptococcus pneumoniae genetika   metabolismus   MeSH
• zastoupení bazí genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• aminoacyltransferasy MeSH
• bakteriální proteiny MeSH
• faktory virulence MeSH
• GpsB protein, Streptococcus pneumoniae MeSH Prohlížeč
• membránové proteiny MeSH
• penicillin-binding protein 2b, Streptococcus MeSH Prohlížeč
• peptidoglykan MeSH
• proteiny vázající penicilin MeSH