This study aimed to examine the effect of eicosapentaenoic acid (EPA) on skeletal muscle hypertrophy induced by muscle overload and the associated intracellular signaling pathways. Male C57BL/6J mice were randomly assigned to oral treatment with either EPA or corn oil for 6 weeks. After 4 weeks of treatment, the gastrocnemius muscle of the right hindlimb was surgically removed to overload the plantaris and soleus muscles for 1 or 2 weeks. We examined the effect of EPA on the signaling pathway associated with protein synthesis using the soleus muscles. According to our analysis of the compensatory muscle growth, EPA administration enhanced hypertrophy of the soleus muscle but not hypertrophy of the plantaris muscle. Nevertheless, EPA administration did not enhance the expression or phosphorylation of Akt, mechanistic target of rapamycin (mTOR), or S6 kinase (S6K) in the soleus muscle. In conclusion, EPA enhances skeletal muscle hypertrophy, which can be independent of changes in the AKT-mTOR-S6K pathway.

• MeSH
• fosforylace MeSH
• hypertrofie chemicky indukované   metabolismus   patologie   MeSH
• kosterní svaly účinky léků   metabolismus   MeSH
• kyselina eikosapentaenová terapeutické užití   MeSH
• modely u zvířat MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• protoonkogenní proteiny c-akt metabolismus   MeSH
• signální transdukce MeSH
• TOR serin-threoninkinasy metabolismus   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• Akt1 protein, mouse MeSH Prohlížeč
• kyselina eikosapentaenová MeSH
• mTOR protein, mouse MeSH Prohlížeč
• protoonkogenní proteiny c-akt MeSH
• TOR serin-threoninkinasy MeSH

Acute myocardial infarction (AMI) is one of the leading causes of death among adults in older age. Understanding mechanisms how organism responds to ischemia is essential for the ischemic patient's prevention and treatment. Despite the great prevalence and incidence only a small number of studies utilize a metabolomic approach to describe AMI condition. Recent studies have shown the impact of metabolites on epigenetic changes, in these studies plasma metabolites were related to neurological outcome of the patients making metabolomic studies increasingly interesting. The aim of this study was to describe metabolomic response of an organism to ischemic stress through the changes in energetic metabolites and aminoacids in blood plasma in patients overcoming acute myocardial infarction. Blood plasma from patients in the first 12 h after onset of chest pain was collected and compared with volunteers without any history of ischemic diseases via NMR spectroscopy. Lowered plasma levels of pyruvate, alanine, glutamine and neurotransmitter precursors tyrosine and tryptophan were found. Further, we observed increased plasma levels of 3-hydroxybutyrate and acetoacetate in balance with decreased level of lipoproteins fraction, suggesting the ongoing ketonic state of an organism. Discriminatory analysis showed very promising performance where compounds: lipoproteins, alanine, pyruvate, glutamine, tryptophan and 3-hydroxybutyrate were of the highest discriminatory power with feasibility of successful statistical discrimination.

• MeSH
• acetoacetáty krev   MeSH
• biologické markery krev   MeSH
• bolesti na hrudi krev   patofyziologie   MeSH
• infarkt myokardu krev   diagnóza   MeSH
• kyselina 3-hydroxymáselná krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lipoproteiny krev   MeSH
• magnetická rezonanční spektroskopie metody   MeSH
• metabolom MeSH
• ROC křivka MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• acetoacetáty MeSH
• acetoacetic acid MeSH Prohlížeč
• biologické markery MeSH
• kyselina 3-hydroxymáselná MeSH
• lipoproteiny MeSH