Most cited article - PubMed ID 28937259
Cardiac morphofunctional characteristics of transgenic rats with overexpression of the bradykinin B1 receptor in the endothelium
The kinin receptors are classically involved in inflammation, pain and sepsis. The effects of the kinin B1 receptor agonist des-Arg9-bradykinin (DBK) and lipopolysaccharide (LPS) were investigated by comparing the membrane potential responses of aortic rings from transgenic rats overexpressing the kinin B1 receptor (B1R) in the endothelium (TGR(Tie2B1)) and Sprague Dawley (SD) rats. No difference in the resting membrane potential in the aorta's smooth muscle from the transgenic and SD rats was observed. The aorta rings from SD rats hyperpolarized only to LPS but not to DBK, whereas the aorta rings from TGR(Tie2B1) responded by the administration of both drugs. DBK and LPS responses were inhibited by the B1 receptor antagonist R715 and by iberiotoxin in both cases. Thapsigargin induced a hyperpolarization in the smooth muscle of SD rats that was not reversed by R715, but was reversed by iberiotoxin and this hyperpolarization was further augmented by DBK administration. These results show that the model of overexpression of vascular B1 receptors in the TGR(Tie2B1) rats represent a good model to study the role of functional B1 receptors in the absence of any pathological stimulus. The data also show that KCa channels are the final mediators of the hyperpolarizing responses to DBK and LPS. In addition, we suggest an interaction between the B1R and TLR4, since the hyperpolarization induced by LPS could be abolished in the presence of R715.
- MeSH
- Aorta MeSH
- Bradykinin * pharmacology MeSH
- Endothelium, Vascular MeSH
- Rats MeSH
- Lipopolysaccharides pharmacology MeSH
- Membrane Potentials MeSH
- Rats, Sprague-Dawley MeSH
- Rats, Transgenic MeSH
- Receptor, Bradykinin B1 * genetics MeSH
- In Vitro Techniques MeSH
- Thapsigargin pharmacology MeSH
- Toll-Like Receptor 4 MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Bradykinin * MeSH
- Lipopolysaccharides MeSH
- Receptor, Bradykinin B1 * MeSH
- Thapsigargin MeSH
- Toll-Like Receptor 4 MeSH
