Nejvíce citovaný článek - PubMed ID 29016942
BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana
Cell polarity is a fundamental feature of all multicellular organisms. PIN auxin transporters are important cell polarity markers that play crucial roles in a plethora of developmental processes in plants. Here, to identify components involved in cell polarity establishment and maintenance in plants, we performed a forward genetic screening of PIN2:PIN1-HA;pin2 Arabidopsis (Arabidopsis thaliana) plants, which ectopically express predominantly basally localized PIN1 in root epidermal cells, leading to agravitropic root growth. We identified the regulator of PIN polarity 12 (repp12) mutation, which restored gravitropic root growth and caused a switch in PIN1-HA polarity from the basal to apical side of root epidermal cells. Next Generation Sequencing and complementation experiments established the causative mutation of repp12 as a single amino acid exchange in Aminophospholipid ATPase3 (ALA3), a phospholipid flippase predicted to function in vesicle formation. repp12 and ala3 T-DNA mutants show defects in many auxin-regulated processes, asymmetric auxin distribution, and PIN trafficking. Analysis of quintuple and sextuple mutants confirmed the crucial roles of ALA proteins in regulating plant development as well as PIN trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with the ADP ribosylation factor GTPase exchange factors GNOM and BIG3 in regulating PIN polarity, trafficking, and auxin-mediated development.
- MeSH
- ADP-ribosylační faktory metabolismus MeSH
- Arabidopsis účinky léků metabolismus MeSH
- biologický transport účinky léků MeSH
- brefeldin A farmakologie MeSH
- buněčná membrána účinky léků metabolismus MeSH
- genetická epistáze účinky léků MeSH
- GTP-fosfohydrolasy metabolismus MeSH
- kyseliny indoloctové metabolismus MeSH
- mutace genetika MeSH
- proteiny huseníčku metabolismus MeSH
- proteiny přenášející fosfolipidy metabolismus MeSH
- tabák metabolismus MeSH
- trans-Golgiho síť účinky léků metabolismus MeSH
- vazba proteinů účinky léků MeSH
- výměnné faktory guaninnukleotidů metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ADP-ribosylační faktory MeSH
- brefeldin A MeSH
- GTP-fosfohydrolasy MeSH
- kyseliny indoloctové MeSH
- proteiny huseníčku MeSH
- proteiny přenášející fosfolipidy MeSH
- výměnné faktory guaninnukleotidů MeSH
