Gamma-glutamyltransferase (GGT), a marker of liver disease, has been shown to be associated with increased risk of diabetes and relative insulin secretion deficiency. However, the mechanism of hepatic Ggt regulation has not been explored fully. In this study, we made a concerted effort to understand the mechanism by investigating the effects of acetylation of histones H3 and H4, and bindings of histone acetyltransferases, CREB binding protein (CBP) and p300, at the Ggt promoter on the regulation of the expression of Ggt gene in the livers of streptozotocin (STZ)-induced moderate hypoinsulinemia rat model. The rats treated with STZ showed remarkably higher serum GGT level and hepatic Ggt/GGT expression than the untreated control rats. Furthermore, the acetylation of histones H3 and H4, and the binding of CBP not p300 at the Ggt promoter regions were significantly higher in the livers of STZ rats than those of the control rats. These results suggest that an enhanced hepatic expression of Ggt is associated with increased acetylation of histones H3 and H4 and CBP binding at the Ggt promoter in STZ-induced moderate hypoinsulinemic rats.

• MeSH
• Acetylation MeSH
• Diabetes Mellitus, Experimental enzymology   genetics   MeSH
• gamma-Glutamyltransferase biosynthesis   genetics   MeSH
• Histone Acetyltransferases metabolism   MeSH
• Histones metabolism   MeSH
• Liver enzymology   MeSH
• Rats MeSH
• Rats, Wistar MeSH
• Promoter Regions, Genetic MeSH
• E1A-Associated p300 Protein metabolism   MeSH
• CREB-Binding Protein metabolism   MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• CREBBP protein, human MeSH Browser
• Crebbp protein, rat MeSH Browser
• Ep300 protein, rat MeSH Browser
• gamma-Glutamyltransferase MeSH
• Histone Acetyltransferases MeSH
• Histones MeSH
• E1A-Associated p300 Protein MeSH
• CREB-Binding Protein MeSH