The expression of vimentin and the phosphorylated variant of high molecular weight neurofilament protein (NF-H) was studied in developing human fetal dorsal root ganglia and spinal cord. The technique used for examination of cryosections was double-label fluorescence with monoclonal antibodies. Both proteins were present in the nerve fibres inside the ganglia of 6- and 8-week-old embryos. During further development the expression of vimentin continued to increase in the satellite cells, but was found to be decreasing in the ganglion cells. Phosphorylated NF-H was found in the processes of ganglion cells, as well as in the perikarya at all developmental stages. In the spinal cord of 6- and 8-week-old embryos, phosphorylated NF-H protein was found in the longitudinal fibres of the marginal layer and in processes of the mantle zone; some of the fibres also contained vimentin. Later the co-expression of the two proteins ceased and vimentin was found only in glial and mesenchymal derivatives. Phosphorylated NF-H was located, at all developmental stages, in the axons of both white and grey matter, but not in the neuronal perikarya. The results indicate that phosphorylation of the NF-H in human dorsal root ganglia starts in the perikarya of the ganglion cells while in the ganglion cells of the spinal cord it takes place in the axons.

• MeSH
• fosforylace MeSH
• gestační stáří MeSH
• lidé MeSH
• mícha embryologie   metabolismus   MeSH
• molekulová hmotnost MeSH
• monoklonální protilátky MeSH
• neurofilamentové proteiny chemie   imunologie   metabolismus   MeSH
• proteiny intermediálních filament metabolismus   MeSH
• specificita protilátek MeSH
• spinální ganglia embryologie   metabolismus   MeSH
• vimentin metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• monoklonální protilátky MeSH
• neurofilament protein H MeSH Prohlížeč
• neurofilamentové proteiny MeSH
• proteiny intermediálních filament MeSH
• vimentin MeSH

Expression of intermediate filament proteins was studied in human developing spinal cord using immunoperoxidase and double-label immunofluorescence methods with monoclonal antibodies to vimentin and glial fibrillary acidic protein (GFAP). Vimentin was found in the processes of radial glial cells in 6-week embryos, while GFAP appeared in vimentin-positive astroglial cells at 8-10 weeks. GFAP and vimentin were present in approximately equal amounts in differentiating astrocytes in 23-week spinal cord. In 30-week fetuses, astrocytes reacted strongly for GFAP, while both the reaction intensity and the number of vimentin-positive cells fluctuated predominantly in the grey matter. No clear-cut transition from vimentin to GFAP was noticed during the development of astrocytes. The majority of ependymal cells in 23-week fetuses contained vimentin but only a few of them reacted for GFAP. The expression of vimentin continued during the whole development of the ependymal layer, in contrast to the reactivity for GFAP which disappeared between the 30th week and term.

• MeSH
• barvení a značení MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• exprese genu MeSH
• fluorescenční mikroskopie MeSH
• fluorescenční protilátková technika MeSH
• gestační stáří MeSH
• gliový fibrilární kyselý protein genetika   imunologie   MeSH
• lidé MeSH
• mícha embryologie   metabolismus   MeSH
• monoklonální protilátky MeSH
• těhotenství MeSH
• vimentin genetika   imunologie   MeSH
• Check Tag
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• gliový fibrilární kyselý protein MeSH
• monoklonální protilátky MeSH
• vimentin MeSH