Stimulation of polymorphonuclear leukocytes with kallikrein demonstrated that enzyme acts selectively on the release of lysosomal enzymes of these cells. The release of collagenase, similarly to the release of lysozyme into the incubation medium increased proportionally to kallikrein concentration and the duration of incubation. Kallikrein had a small effect on beta-glucuronidase secretion. No effect on cytoplasm lactate dehydrogenase release was detected. These results suggest that kallikrein, as a soluble stimulus, predominantly induces degranulation of specific granules containing collagenase capable of degrading the connective tissue. Secretion of lysozyme and collagenase requires the presence of active kallikrein. Soybean trypsin inhibitor diminished the enzyme release.

• MeSH
• glukuronidasa metabolismus   MeSH
• kalikreiny krev   farmakologie   MeSH
• lidé MeSH
• lyzozomy účinky léků   enzymologie   MeSH
• mikrobiální kolagenasa metabolismus   MeSH
• muramidasa metabolismus   MeSH
• neutrofily účinky léků   enzymologie   ultrastruktura   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• glukuronidasa MeSH
• kalikreiny MeSH
• mikrobiální kolagenasa MeSH
• muramidasa MeSH