Metyrapone, a potent cytochrome P-450 inhibitor, added at 9 mM to a submerged culture of Streptomyces cinnamonensis caused partial inhibition of total monensin biosynthesis and coproduction of new metabolites, 26-deoxymonensins A and B. The latter was isolated as its 25-O-methyl derivative. Metyrapone was simultaneously reduced to metyrapol. All of these compounds were identified by nuclear magnetic resonance spectroscopy and mass spectrometry.

• MeSH
• Anti-Bacterial Agents biosynthesis   chemistry   isolation & purification   MeSH
• Mass Spectrometry MeSH
• Magnetic Resonance Spectroscopy MeSH
• Metyrapone analogs & derivatives   metabolism   MeSH
• Monensin analogs & derivatives   biosynthesis   chemistry   isolation & purification   MeSH
• Pyridines metabolism   pharmacology   MeSH
• Streptomyces drug effects   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• 26-deoxymonensin A MeSH Browser
• 26-deoxymonensin B MeSH Browser
• Anti-Bacterial Agents MeSH
• metapyrone MeSH Browser
• metyrapol MeSH Browser
• Metyrapone MeSH
• Monensin MeSH
• Pyridines MeSH

Variants resistant to propionate were prepared from a mutant strain of Streptomyces cinnamonensis producing predominantly monensin A. Using selected resistants the production of monensins (in media with higher concentrations of propionate) was examined. Stimulation of monensin synthesis by propionate was observed with 70% of the resistants studied. Propionate did not influence the ratio between monensin A and B production.

• MeSH
• Drug Resistance, Microbial MeSH
• Monensin biosynthesis   MeSH
• Mutation MeSH
• Propionates pharmacology   MeSH
• Streptomyces metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Monensin MeSH
• Propionates MeSH