Monitoring West Nile virus (WNV) and Usutu virus (USUV) activity now has the highest priority among mosquito-borne pathogenic viruses circulating in the European Union. This study documents a first time detection and the co-circulation of WNV lineage-2 (with the minimal prevalence of 0.46%) and USUV clade Europe 2 (with the minimal prevalence of 0.25%) in mosquitoes from the same habitat of south-western Slovakia and underlines necessity to perform rigorous surveillance in birds, mosquitoes, horses and humans in that country.

• Keywords
• Culex spp., Usutu virus, West Nile fever, West Nile virus, mosquitoes, surveillance,
• MeSH
• Culex virology   MeSH
• Culicidae virology   MeSH
• Ecosystem MeSH
• Flavivirus genetics   isolation & purification   MeSH
• Phylogeny MeSH
• Flavivirus Infections epidemiology   veterinary   MeSH
• Mosquito Vectors virology   MeSH
• Horses virology   MeSH
• Humans MeSH
• Prevalence MeSH
• Birds virology   MeSH
• Public Health MeSH
• West Nile virus genetics   isolation & purification   MeSH
• West Nile Fever epidemiology   veterinary   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Female MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Slovakia epidemiology   MeSH