The extracellular level of brefeldin A fluctuates with the length of malonate inhibition. Following treatment with malonate, mycelial multiplication as opposed to brefeldin A formation, was preferentially increased in the maleate, fumarate, succinate, citrate, methyl palmitate and glucose replacement cultures. Competitive maleate--malonate, fumarate--malonate, succinate--malonate and citrate--malonate-inhibited replacement cultures gave significantly higher mycelial and brefeldin A yields than the sole malonate-inhibited replacement cultures.

• MeSH
• Anti-Bacterial Agents biosynthesis   MeSH
• Brefeldin A MeSH
• Cyclopentanes biosynthesis   MeSH
• Carboxylic Acids metabolism   MeSH
• Malonates pharmacology   MeSH
• Mitosporic Fungi drug effects   growth & development   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Brefeldin A MeSH
• Cyclopentanes MeSH
• Carboxylic Acids MeSH
• Malonates MeSH

Extracellular brefeldin A was detected in 4% glucose--peptone--mineral salts cultures of Curvularia lunata at the start of the exponential growth phase. Some fluctuations in brefeldin A levels occurred during the exponential growth phase followed by a significant reduction in level at the stationary growth phase. Broth glucose levels decreased according to a sigmoid relationship with time whereas broth pH remained fairly constant during the exponential growth phase followed by a gradual increase into the stationary growth phase. Mycelial brefeldin A levels were low throughout the various growth phases. The principal fatty acids present in decreasing order during the exponential growth phase were linoleic, oleic and palmitic acids. However, the content of linoleic acid was significantly reduced at the onset and during the stationary growth phase.

• MeSH
• Anti-Bacterial Agents biosynthesis   MeSH
• Brefeldin A MeSH
• Cyclopentanes biosynthesis   MeSH
• Glucose metabolism   MeSH
• Hydrogen-Ion Concentration MeSH
• Culture Media MeSH
• Fatty Acids, Nonesterified metabolism   MeSH
• Mitosporic Fungi growth & development   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Brefeldin A MeSH
• Cyclopentanes MeSH
• Glucose MeSH
• Culture Media MeSH
• Fatty Acids, Nonesterified MeSH