Streptomyces strain 3B constitutively secreted collagenolytic enzymes during the post-exponential growth phase. Purification is described here leading to two collagenases (I and II) with specific activity of 3350 and 3600 U/mg, respectively, the highest activity obtained as yet for any streptomycete collagenase. Analysis of the purified enzymes by the method of zymography revealed that both I and II were homogeneous, with molar mass 116 and 97 kDa, respectively. Both collagenases were identical in their pH (7.5) and temperature optimum (37 degrees C). The inhibition profile of the enzymes by EDTA and 1,10-phenanthroline confirmed these enzymes to be metalloproteinases. By testing the activity with insoluble collagen, acid soluble collagen, gelatin, casein, elastin and Pz-PLGPR it was established that I and II are very specific for insoluble collagen and gelatin, showing a high activity toward acid soluble collagen and Pz-PLGPR. However, collagenases I and II failed to hydrolyze casein and elastin; they belong to true collagenases and resemble the clostridial enzymes.

• MeSH
• Collagenases chemistry   isolation & purification   MeSH
• Hydrogen-Ion Concentration MeSH
• Streptomyces enzymology   MeSH
• Substrate Specificity MeSH
• Temperature MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Collagenases MeSH

A new antifungal antibiotic named acmycin was isolated from a soil streptomycete. Detailed comparative taxonomic studies showed that the organism differed from three related species of streptomycetes. The organism was referred to as Streptomyces sp. AC2. The isolated antibiotic appears to be of polyene nature.

• MeSH
• Antifungal Agents analysis   biosynthesis   MeSH
• Chromatography MeSH
• Hydrogen-Ion Concentration MeSH
• Polyenes analysis   biosynthesis   MeSH
• Soil Microbiology MeSH
• Streptomyces classification   isolation & purification   metabolism   MeSH
• Hot Temperature MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• acmycin MeSH Browser
• Antifungal Agents MeSH
• Polyenes MeSH

• MeSH
• Cations pharmacology   MeSH
• Collagen metabolism   MeSH
• Hydrogen-Ion Concentration MeSH
• Microbial Collagenase metabolism   MeSH
• Soil Microbiology * MeSH
• Streptomyces enzymology   MeSH
• Temperature MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Cations MeSH
• Collagen MeSH
• Microbial Collagenase MeSH