Mycelium of Streptomyces cinnamonensis mutant strains cultivated in a synthetic medium with glycine produced protoplasts after lysis of cell walls with lysozyme. The protoplast yield was up to 95%. The protoplasts could revert and mycelial forms were thus regenerated. In a sucrose-containing medium the protoplasts stored at 4 degrees C were stable for 2 d.

• MeSH
• Glycine MeSH
• Culture Media MeSH
• Muramidase MeSH
• Mutation MeSH
• Mycology methods   MeSH
• Protoplasts cytology   physiology   MeSH
• Streptomyces cytology   genetics   physiology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Glycine MeSH
• Culture Media MeSH
• Muramidase MeSH

Analytical and preparative high-performance liquid chromatography of 3 phenazines and furonaphthoquinone derivative on reversed-phase column are described. The mobile phase was methanol and water. The injected amount of the mixture was about 30 mg for a preparative chromatographic run requiring 80 min. Substances were detected directly in the column effluent by UV detection.

• MeSH
• Phenazines isolation & purification   MeSH
• Furans isolation & purification   MeSH
• Naphthoquinones isolation & purification   MeSH
• Streptomyces analysis   MeSH
• Chromatography, High Pressure Liquid * MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Phenazines MeSH
• Furans MeSH
• Naphthoquinones MeSH

Substances studied at this department in 1954-1983 are reviewed; a total of 226 compounds are characterized in a tabular form. They include natural compounds as well as those prepared by biotransformation, by semisynthetic and synthetic methods.

• MeSH
• Anti-Bacterial Agents isolation & purification   MeSH
• Microbiology MeSH
• Chemistry, Organic * MeSH
• Organic Chemistry Phenomena MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH