Mycelia of a low- and a high-production strain of Streptomyces aureofaciens were converted into protoplasts and divided into five subcellular fractions in order to localize exopolyphosphatases (EC 3.6.1.11), triphosphatase (EC 3.6.1.25), inorganic diphosphatase (EC 3.6.1.1), apyrase (EC 3.6.1.5) and glucokinase (EC 2.7.1.2). The highest specific activity of enzymes hydrolyzing polyphosphates was found in cytoplasmic vesicles and membranes. Triphosphatase was detected in the periplasmic fraction. Periplasmic vesicles and cytoplasm exhibited a high activity of diphosphatase. Apyrase was found mainly in the fractions of membranes and cytoplasmic vesicles. Glucokinase was a cytoplasmic enzyme. The enzymes were released from membrane structures into cytoplasm or periplasmic space if benzyl thiocyanate (10 microM) was present in the growth medium.

• MeSH
• draslík farmakologie   MeSH
• fosfatasy metabolismus   MeSH
• glukokinasa metabolismus   MeSH
• hořčík farmakologie   MeSH
• kationty MeSH
• kinetika MeSH
• sodík farmakologie   MeSH
• Streptomyces aureofaciens účinky léků   enzymologie   MeSH
• subcelulární frakce enzymologie   MeSH
• thiokyanatany farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• benzyl thiocyanate MeSH Prohlížeč
• draslík MeSH
• fosfatasy MeSH
• glukokinasa MeSH
• hořčík MeSH
• kationty MeSH
• sodík MeSH
• thiokyanatany MeSH

ATP diphosphohydrolase activity and inorganic pyrophosphatase reached two maxima during cultivation of the low- and high-producing variant of Streptomyces aureofaciens under conditions of phosphate limitation, i.e. after 30 and 70 h of cultivation. Increased levels of inorganic phosphate in a medium inhibitory to biosynthesis of chlortetracycline markedly decreased the levels of both enzymes. The ATP diphosphohydrolase activity was detected both in the supernatant and membrane fractions of the cell-free preparation of the mycelium.

• MeSH
• apyrasa metabolismus   MeSH
• chlortetracyklin biosyntéza   MeSH
• fosfatasy metabolismus   MeSH
• fosfáty farmakologie   MeSH
• kultivační média MeSH
• membrány enzymologie   MeSH
• pyrofosfatasy metabolismus   MeSH
• Streptomyces enzymologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• apyrasa MeSH
• chlortetracyklin MeSH
• fosfatasy MeSH
• fosfáty MeSH
• kultivační média MeSH
• pyrofosfatasy MeSH