Raisins are one of the most nutritious dried fruits in the world. The occurrence of ochratoxin A (OTA) in raisins results from contamination and growth of ochratoxigenic fungi and short-term humid weather during drying. The estimation of the dietary exposure dose of OTA from raisins for the Czech population (children, adult) is presented. The risk of acute toxic effect of OTA was in the Czech Republic usually considered to be minimal. The risk of late toxic effects (particularly carcinogenic risk) after ingestion of very low single or repeated OTA doses from foodstuffs is very important.

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Mycotoxins are naturally occurring secondary metabolites produced by several toxigenic microscopic fungi on a variety of crops, especially cereal grains and further foodstuffs. Series of experimental research projects on the determination of mycotoxins (aflatoxins, cyclopiazonic acid, ochratoxin A, patulin, deoxynivalenol, fumonisin B1, T-2 toxin, zearalenone, sterigmatocystin, alternaria toxins) in several foods were realized in the National Reference Centre for Microscopic Fungi and Mycotoxins in the 90(th) years. The aim of our work was an estimation of dietary exposure to mycotoxins and risk assessment. The method of a solid phase extraction (SPE), liquid - liquid extraction and immunoaffinity chromatography (f. e. R-Biopharm, VICAM) were used to elaborate for sample analyses of mycotoxins in our projects. The mycotoxins were detected most frequently by chromatographic methods (HPTLC, HPLC, GC) and immunochemical methods (ELISA). Average dietary exposure has been calculated by multiplying of concentration data for specific foods with their consumption rates per 1 kg of b. w. per day. The estimation of the dietary exposure dose of mycotoxins for the Czech population is presented.

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