The authors tested the fungicide effect of different biocides by the diffusion method. Its principle involves pipeting samples of the tested fungicide preparations into pits in an agar medium on Petri dishes. The agar is inoculated over the whole surface with spores of fungi. It is incubated at 28 degrees C for 2 to 3 days. The size of the inhibition zones in the increments of fungi round the pits with the preparation are recorded. The authors discuss the suitability of this method for different types of preparations with regard to the results they assembled.
An immunochemical method was proposed for simultaneous determination of aflatoxin and ochratoxin A with the use of mixed solutions of the following reagents: standards of both mycotoxins, antiserums against the mycotoxins, and radioligands of 125I-aflatoxin B1 and 125I-ochratoxin A. The result of the analytical procedure is the value of concentration of the aflatoxin + ochratoxin A sum in the sample. The procedure needs half the amount of reagents as separate determination of each of the two mycotoxin, and is far less laborious. The proposed simultaneous immunoanalysis is suitable for large-are inspection of grain and feed safety from the viewpoint of aflatoxin and ochratoxin A levels.
- MeSH
- aflatoxiny analýza MeSH
- jedlá semena chemie MeSH
- krmivo pro zvířata analýza MeSH
- ochratoxiny analýza MeSH
- radioimunoanalýza metody MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- aflatoxiny MeSH
- ochratoxin A MeSH Prohlížeč
- ochratoxiny MeSH
In order to facilitate a practical large-scale introduction of the radioimmunochemical control of aflatoxin B1 contamination in cereals and feeds a new method of the preparation of sample extracts was verified. In comparison with the procedure used until now when water-nonmiscible chloroform must be evaporated from a chloroform extract and the solids must be dissolved in acetone, while using the tested method the sample is extracted by methyl alcohol or acetone and the extract is diluted by a buffer before the radioimmunoanalysis. This new method of sample extract preparation displays aflatoxin B1 recovery comparable to that of the older method (83-90% for concentrations of 2-20 micrograms per kg), the worse theoretical detection limit (1 microgram per kg vs. 0.2 microgram per kg) but it prevents the result distortion by interfering substances and it makes the whole analytical procedure simpler and expeditious. Radioimmunochemically aflatoxin was quantified by means of commercially produced analytical kits developed in one institute at Kosice.
- MeSH
- aflatoxin B1 MeSH
- aflatoxiny analýza MeSH
- jedlá semena analýza MeSH
- karcinogeny analýza MeSH
- krmivo pro zvířata analýza MeSH
- radioimunoanalýza přístrojové vybavení metody MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- aflatoxin B1 MeSH
- aflatoxiny MeSH
- karcinogeny MeSH
