OBJECTIVE: To study the effect of the stress of chronic food restriction on the development of adjuvant arthritis in Long Evans male rats. METHODS: Four groups of animals were compared: non-treated control (C) and arthritic (AA) rats, both with free access to food and water and two analogous groups with a 40% food restriction, i.e. non-treated (FR) and arthritic (AA-FR) animals. All rats were killed 22 days following the injection of complete Freund's adjuvant. The parameters measured were: serum levels of albumin (ALB), nitrate, glucose, insulin, corticosterone (CORT), prolactin (PRL) and PRL mRNA in the adenopituitaries. In addition the activity of gamma-glutamyl transpeptidase (GGTP) was measured in the spleen. The pain threshold was determined by the tailflick method. The body weight of the animals was recorded on day 0, 3, 7, 11, 15 and 18 of the disease. RESULTS: Arthritis caused swelling of the hind paw (2.37 +/- 0.15 ml vs 1.1 +/- 0.05ml in controls, p < 0.01) which was prevented in the AA-FR group (1.44 +/- 0.13 ml, not significant against controls). Arthritis increased serum NO and reduced ALB levels; both changes were significantly restored in the FR-AA group. Food restriction did not alter the activation of GGTP, or the decrease of PRL mRNA observed in the AA group. Serum CORT was elevated in rats with food restriction (15.49 +/- 2.1 vs. 3.7 +/- 0.87 microg/dL) and remained enhanced to the same extent in AA and FR-AA groups. The tailflick latency prolonged in the AA group was reduced by food restriction. CONCLUSION: These results show that 40% food restriction associated with elevated CORT levels mitigated the inflammatory parameters activated during AA.

• MeSH
• adenohypofýza metabolismus   MeSH
• artritida experimentální krev   prevence a kontrola   psychologie   MeSH
• dusičnany krev   MeSH
• gama-glutamyltransferasa metabolismus   MeSH
• inzulin krev   MeSH
• kortikosteron krev   MeSH
• krevní glukóza MeSH
• krysa rodu Rattus MeSH
• měření bolesti MeSH
• messenger RNA metabolismus   MeSH
• potkani Long-Evans MeSH
• potravinová deprivace * MeSH
• práh bolesti psychologie   MeSH
• prolaktin krev   genetika   MeSH
• psychický stres * MeSH
• sérový albumin analýza   MeSH
• sexuální faktory MeSH
• slezina enzymologie   MeSH
• tělesná hmotnost fyziologie   MeSH
• zadní končetina patologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dusičnany MeSH
• gama-glutamyltransferasa MeSH
• inzulin MeSH
• kortikosteron MeSH
• krevní glukóza MeSH
• messenger RNA MeSH
• prolaktin MeSH
• sérový albumin MeSH