Exosomes are small vesicles that are secreted by cells and act as mediators of cell to cell communication. Because of their potential therapeutic significance, important efforts are being made towards characterizing exosomal contents. However, little is known about the mechanisms that govern exosome biogenesis. We have recently shown that the exosomal protein syntenin supports exosome production. Here we identify the small GTPase ADP ribosylation factor 6 (ARF6) and its effector phospholipase D2 (PLD2) as regulators of syntenin exosomes. ARF6 and PLD2 affect exosomes by controlling the budding of intraluminal vesicles (ILVs) into multivesicular bodies (MVBs). ARF6 also controls epidermal growth factor receptor degradation, suggesting a role in degradative MVBs. Yet ARF6 does not affect HIV-1 budding, excluding general effects on Endosomal Sorting Complexes Required for Transport. Our study highlights a novel pathway controlling ILV budding and exosome biogenesis and identifies an unexpected role for ARF6 in late endosomal trafficking.
- MeSH
- ADP-ribosylační faktor 6 MeSH
- ADP-ribosylační faktory genetika metabolismus MeSH
- buněčné linie MeSH
- endozomální třídící komplexy pro transport genetika metabolismus MeSH
- erbB receptory metabolismus MeSH
- exozómy enzymologie genetika metabolismus MeSH
- fosfolipasa D genetika metabolismus MeSH
- HIV infekce genetika metabolismus virologie MeSH
- HIV-1 fyziologie MeSH
- lidé MeSH
- multivezikulární tělíska enzymologie genetika metabolismus MeSH
- proteiny buněčného cyklu genetika metabolismus MeSH
- proteiny vázající vápník genetika metabolismus MeSH
- synteniny genetika metabolismus MeSH
- transport proteinů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- ADP-ribosylační faktor 6 MeSH
- ADP-ribosylační faktory MeSH
- ARF6 protein, human MeSH Prohlížeč
- endozomální třídící komplexy pro transport MeSH
- erbB receptory MeSH
- fosfolipasa D MeSH
- PDCD6IP protein, human MeSH Prohlížeč
- phospholipase D2 MeSH Prohlížeč
- proteiny buněčného cyklu MeSH
- proteiny vázající vápník MeSH
- synteniny MeSH
