1. Cytological diagnosis of changes of the cylindrical epithelium in the endocervix has not been elaborated in such detail as the cytodiagnosis of squamous epithelium of the ectocervix. 2. The original proposal and experience of Vooijs of 1995 for the classification of incipient endocervical lesions corresponds best to histological practice. 3. Cytodiagnosis of endocervical changes will be more accurate if we use for evaluation the cellular pattern from the endocervix, demonstrated on the plates. 4. If we find any cellular abnormalities of the cylindrical epithelium, we recommend a procedure as used in lesions of squamous epithelium a) Low-grade abnormalities should be followed up primarily by cytological methods. If the lesion persists or progresses, histological examination is indicated. Examination of an endocervical lesion is usually not accessible to colposcopy. b) High-grade abnormalities and in particular those with the above described architecture, should be subjected to histological examination. 5. In mixed cylindrical and squamous lesions the procedure depends on the more serious lesion. In mixed lesions or suspected mixed lesions it is an advantage to use histochemical examination for betagalactosidase, the method described by Lojda, which differentiates safely the squamous and cylindrical component and in particular its tendency of malignant reversal.
- MeSH
- cervix uteri patologie MeSH
- lidé MeSH
- nádory děložního čípku diagnóza MeSH
- vaginální stěr * MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
A group of 70 female patients with adenocarcinoma of the uterine cervix treated between 1976 and 1995 at the 2nd Department of Obstetrics and Gynaecology, 1st Faculty of Medicine, Charles University and General Hospital, Prague, was evaluated retrospectively. They represented 8.6% of all patients with cervical cancer in this period. The mean age was 57.9 years and increased according to the clinical stage of disease. Some features of the patient history were similar to those of endometrial cancer. The most frequent symptom was abnormal bleeding (70%). The limited importance of praebioptic diagnostic methods was confirmed. Better treatment results were achieved by the surgical or combined treatment in comparison with radiotherapy alone. From histological types the worst results had a clear cell carcinoma. The recurrence rate was 13.4%. The overall 5-year survival was 43.1% (68% for the stage 1, 40% for the stage 2 and 22% for the stages 3 + 4). The average survival time was 96.1 months.
- MeSH
- adenokarcinom * diagnóza mortalita patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádory děložního čípku * diagnóza mortalita patologie MeSH
- retrospektivní studie MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- MeSH
- hořčík fyziologie terapeutické užití MeSH
- komplikace těhotenství * etiologie patofyziologie MeSH
- lidé MeSH
- nedostatek hořčíku komplikace diagnóza patofyziologie terapie MeSH
- předčasná porodní činnost etiologie patofyziologie MeSH
- těhotenství MeSH
- Check Tag
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- hořčík MeSH
The authors investigated a group of 195 patients from the Centre of Oncological Prevention for human papillomavirus (HPV) in the lower genital tract. The diagnostic set ViraPap/ViraType was used for detection. It is based on double hybridisation of viral DNA and on the use of polyclonal and monoclonal antibodies with chemoluminiscent reaction. The test was totally positive in 23.1% of women. 5.1% tests were positive in the low-risk group of viruses (LR-HPV) and 19.5% in the high risk group (HR-HPV). Precancerous lesions (CIN I-III) or invasive cancer were found in 86.7% of positive tests. In patients with CIN III 37.1% were HPV positive and all patients with invasive cancer were positive. The results support an association between CIN and HPV infections. The possible role of HPV in cancerogenesis is discussed as well as the clinical importance of their detection.
- MeSH
- cervix uteri virologie MeSH
- gynekologická onemocnění diagnóza MeSH
- hybridizace nukleových kyselin MeSH
- infekce onkogenními viry diagnóza MeSH
- infekce papilomavirem diagnóza MeSH
- lidé MeSH
- luminiscenční měření MeSH
- nádory ženských pohlavních orgánů diagnóza MeSH
- Papillomaviridae izolace a purifikace MeSH
- reakce antigenu s protilátkou MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- MeSH
- alfa-fetoproteiny analýza MeSH
- břišní svaly abnormality MeSH
- lidé MeSH
- nemoci plodu diagnóza MeSH
- prenatální diagnóza * MeSH
- pupeční kýla diagnóza MeSH
- spina bifida diagnóza MeSH
- těhotenství MeSH
- ultrasonografie prenatální MeSH
- vrozené vady diagnóza MeSH
- Check Tag
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alfa-fetoproteiny MeSH
1. Evaluation of cytological cervico-vaginal smears by the Bethesda system enlisted cytodiagnostics among important laboratory methods which can be used also in risk pregnancies. 2. Vaginal cytology makes it possible to test at the same time the hormonal situation during pregnancy, which reflects the placental function, and to evaluate also the vaginal biocenosis. 3. The authors provided evidence that the large number of superficial cells on the cytological smear (more than 10%) is associated with low oestriol and pregnandiol levels which are warning signs of the approaching termination of pregnancy. 4. By the action of microorganisms on the vaginal epithelium typical morphological changes develop in the cell nucleus and in the cytoplasm. By polychromatic staining also the causal agents of inflammations and infections threatening the mother and foetus are apparent. 5. The authors assume that cytological examination and evaluation according to the Bethesda system should be included in the complex of antenatal examinations also in women without clinical symptoms of premature delivery or without signs of vaginal infection.
- MeSH
- cervix uteri patologie MeSH
- cytodiagnostika MeSH
- infekční komplikace v těhotenství diagnóza patologie MeSH
- lidé MeSH
- předčasná porodní činnost diagnóza etiologie patologie MeSH
- těhotenství MeSH
- vagina patologie MeSH
- Check Tag
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
The cell mediated immunity was examined by the leukocyte adherence inhibition (LAI) test in 21 patients with CIN III and 146 patients with invasive gynaecologic malignancies before treatment and at 3-month intervals. Our own LDV antigen and organ specific antigens were used. The test was highly specific (95.1%) in comparison with a control group of healthy blood donors. The sensitivity of the test was lower (64.7-86.8%). There was no correlation with the development of disease (remission or relapse). The test in a two-year investigation does not seem suitable for monitoring of disease. Depending the nature of LDV infection, only a longer investigation extending over more years would enable further conclusions.
- MeSH
- antigeny virové imunologie MeSH
- buněčná imunita MeSH
- LDH virus imunologie MeSH
- lidé MeSH
- nádory ženských pohlavních orgánů diagnóza imunologie MeSH
- senzitivita a specificita MeSH
- test inhibice adherence leukocytů * MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny virové MeSH
Dynamic follow-up of cell-mediated immunity in women with malignant neoplasms of the ovary provides more valuable information on the course of the disease than a single value. The properties of leukocytes in patients with malignant ovarian tumours must be followed up on a longitudinal basis, as a signal of an approaching relapse of the disease is apparent sooner than its clinical manifestations. It appears that changes of biophysical mechanisms participate not only in the course of malignant ovarian disease but perhaps also in its genesis.
- MeSH
- lidé MeSH
- nádory vaječníků imunologie patologie MeSH
- test inhibice adherence leukocytů * MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
The objective of the work was to evaluate the importance of antenatal examination of amniotic fluid and foetal blood in case of suspected Rh isoimmunization of the foetus. In 1991-1992 in 16 patients with a rise of the titre of anti-D antibodies to > 1:8 between the 24th and 36th week of gestation 32 punctures of the umbilicus by means of a 22 gauge needle were made under continual ultrasonic control. In two instances intraumbilical transfusion was indicated. The authors revealed that with the rising titre of anti-D antibodies in maternal blood the foetal haematocrit value in the umbilical blood declines. With the rising bilirubin level the haematocrit declines. In foetuses with a haematocrit of < 31% severe forms of jaundice are encountered more frequently with the necessity of long-term phototherapy and exchange transfusion. The authors did not find a correlation between the haematocrit of foetal blood and the bilirubinoid concentration in amniotic fluid, assessed by Liley's method. Foetuses with a haematocrit higher than 31% are not threatened by severe forms of jaundice and therefore the authors do not use transfusions in these foetuses. Based on hitherto assembled experience, the authors confirmed that cordocentesis is associated with a comparable risk as amniocentesis but provides more accurate information on the state of the foetus.
- MeSH
- amniocentéza MeSH
- fetální krev MeSH
- hematokrit MeSH
- hemolytická nemoc plodu a novorozence diagnóza terapie MeSH
- intrauterinní krevní transfuze * MeSH
- lidé MeSH
- novorozenec MeSH
- Rh izoimunizace * MeSH
- těhotenství MeSH
- Check Tag
- lidé MeSH
- novorozenec MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
