Interleukin-1α (IL-1α) and Annexin A2 (AnxA2) are pleiotropic molecules with both intracellular and extracellular roles. They share several characteristics including unconventional secretion aided by S100 proteins, anchoring of the externalized proteins at the outer surface of the plasma membrane and response to oxidative stress. Although IL-1α and AnxA2 have been implicated in a variety of biological processes, including cancer, little is known about the mechanisms of their cellular release. In the present study, employing the non-cancerous breast epithelial MCF10A cells, we demonstrate that IL-1α and AnxA2 establish a close association in response to oxidative stress. Stress conditions lead to translocation of both proteins towards lamellipodia rich in vimentin and association of full-length IL-1α and Tyr23 phosphorylated AnxA2 with the plasma membrane at peripheral sites depleted of F-actin. Notably, membrane-associated IL-1α and AnxA2 preferentially localize to the outer edges of the MCF10A cell islands, suggesting that the two proteins participate in the communication of these epithelial cells with their neighboring cells. Similarly, in U2OS osteosarcoma cell line both endogenous IL-1α and transiently produced IL-1α/EGFP associate with the plasma membrane. While benign MFC10A cells present membrane-associated IL-1α and AnxA2 at the edges of their cell islands, the aggressive cancerous U2OS cells communicate in such manner also with distant cells.

• Klíčová slova
• Annexin A2, Interleukin-1α, MCF10A, Oxidative stress, Unconventional secretion,
• MeSH
• aktiny metabolismus   MeSH
• annexin A2 metabolismus   MeSH
• buněčná membrána metabolismus   MeSH
• epitelové buňky metabolismus   MeSH
• fosforylace fyziologie   MeSH
• interleukin-1alfa metabolismus   MeSH
• lidé MeSH
• nádorové buněčné linie MeSH
• oxidační stres fyziologie   MeSH
• proteiny S100 metabolismus   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aktiny MeSH
• annexin A2 MeSH
• ANXA2 protein, human MeSH Prohlížeč
• IL1A protein, human MeSH Prohlížeč
• interleukin-1alfa MeSH
• proteiny S100 MeSH