Current techniques for monitoring GABA (γ-aminobutyric acid), the primary inhibitory neurotransmitter in vertebrates, cannot follow transients in intact neural circuits. To develop a GABA sensor, we applied the design principles used to create the fluorescent glutamate receptor iGluSnFR. We used a protein derived from a previously unsequenced Pseudomonas fluorescens strain and performed structure-guided mutagenesis and library screening to obtain intensity-based GABA sensing fluorescence reporter (iGABASnFR) variants. iGABASnFR is genetically encoded, detects GABA release evoked by electric stimulation of afferent fibers in acute brain slices and produces readily detectable fluorescence increases in vivo in mice and zebrafish. We applied iGABASnFR to track mitochondrial GABA content and its modulation by an anticonvulsant, swimming-evoked, GABA-mediated transmission in zebrafish cerebellum, GABA release events during interictal spikes and seizures in awake mice, and found that GABA-mediated tone decreases during isoflurane anesthesia.

• MeSH
• anestezie MeSH
• biosenzitivní techniky metody   MeSH
• dánio pruhované MeSH
• GABA metabolismus   MeSH
• geneticky modifikovaná zvířata MeSH
• hipokampus metabolismus   MeSH
• krysa rodu Rattus MeSH
• molekulární zobrazování metody   MeSH
• mozek metabolismus   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• neurony metabolismus   MeSH
• potkani Sprague-Dawley MeSH
• záchvaty metabolismus   patologie   MeSH
• zelené fluorescenční proteiny genetika   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• GABA MeSH
• zelené fluorescenční proteiny MeSH