Infective endocarditis (IE) is a life-threatening disease, with its mortality rate varying depending on the infectious agent. Streptococci are among the most common causes of infective endocarditis. However, Streptococcus vestibularis has rarely been associated with human infections, typically affecting patients with underlying conditions such as immunosuppressive diseases, valve replacement, rheumatic heart disease, and hemodialysis. We present the case of a 26-year-old man who presented with fever, unanticipated weight loss, and fatigue. Although no typical risk factors for infective endocarditis were identified at admission, transesophageal echocardiography revealed a bicuspid aortic valve with calcification, paravalvular aortic abscess formation, and vegetations on the anterior leaflet of the mitral valve. Blood cultures grew S. vestibularis, which was initially sensitive to benzylpenicillin but developed emergent resistance on the third day of the antibiotic treatment. Subsequently, ceftriaxone therapy was initiated, and blood cultures became sterile on day 10. The patient eventually underwent aortic valve replacement. We report the first known case of native aortic and mitral valve endocarditis caused by S. vestibularis, accompanied by a paravalvular abscess around the native aortic valve, in a patient who had no typical risk factors for infective endocarditis, except for a bicuspid aortic valve.

• Klíčová slova
• Bicuspid aortic valves, Infective endocarditis, Periannular abscesses, Streptococcus vestibularis,
• MeSH
• aortální chlopeň * MeSH
• bakteriální endokarditida * diagnóza   mikrobiologie   komplikace   MeSH
• dospělí MeSH
• lidé MeSH
• mitrální chlopeň * MeSH
• Streptococcus * izolace a purifikace   MeSH
• streptokokové infekce * diagnóza   komplikace   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

Permeability is an important molecular property in drug discovery, as it co-determines pharmacokinetics whenever a drug crosses the phospholipid bilayer, e.g., into the cell, in the gastrointestinal tract, or across the blood-brain barrier. Many methods for the determination of permeability have been developed, including cell line assays (CACO-2 and MDCK), cell-free model systems like parallel artificial membrane permeability assay (PAMPA) mimicking, e.g., gastrointestinal epithelia or the skin, as well as the black lipid membrane (BLM) and submicrometer liposomes. Furthermore, many in silico approaches have been developed for permeability prediction: meta-analysis of publicly available databases for permeability data (MolMeDB and ChEMBL) was performed to establish their usability. Four experimental and two computational methods were evaluated. It was shown that repeatability of the reported permeability measurement is not great even for the same method. For the PAMPA method, two different permeabilities are reported: intrinsic and apparent. They can vary in degrees of magnitude; thus, we suggest being extra cautious using literature data on permeability. When we compared data for the same molecules using different methods, the best agreement was between cell-based methods and between BLM and computational methods. Existence of unstirred water layer (UWL) permeability limits the data agreement between cell-based methods (and apparent PAMPA) with data that are not limited by UWL permeability (computational methods, BLM, intrinsic PAMPA). Therefore, different methods have different limitations. Cell-based methods provide results only in a small range of permeabilities (-8 to -4 in cm/s), and computational methods can predict a wider range of permeabilities beyond physical limitations, but their precision is therefore limited. BLM with liposomes can be used for both fast and slow permeating molecules, but its usage is more complicated than standard transwell techniques. To sum up, when working with in-house measured or published permeability data, we recommend caution in interpreting and combining them.

• Klíčová slova
• BLM, CACO-2, COSMOperm, MDCK, MolMeDB, PAMPA, PerMM, liposome, membrane, permeability,
• MeSH
• buňky MDCK MeSH
• Caco-2 buňky MeSH
• hematoencefalická bariéra metabolismus   MeSH
• lidé MeSH
• liposomy * chemie   MeSH
• membrány umělé MeSH
• permeabilita buněčné membrány fyziologie   MeSH
• permeabilita * MeSH
• psi MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• psi MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• metaanalýza MeSH
• Názvy látek
• liposomy * MeSH
• membrány umělé MeSH

Temporomandibular disorders (TMDs) are conditions with multifactorial etiology and complex treatment. Among the non-invasive therapeutic possibilities for these conditions is the Front Plateau, a partial anterior plate made from colourless self-curing acrylic resin. It is a simple procedure that can be carried out in a single clinical section promoting muscle relaxation to reduce symptoms associated with TMDs. This study aims to report a prospective, consecutive, single-centric case series to evaluate the Front Plateau's effectiveness in patients with temporomandibular disorders. A questionnaire adapted from the Research Diagnostic Criteria for TMDs was used and 4 patients were treated with the Front Plateau plaque. Patients were monitored after 5 and 9 months, respectively, after starting to use the Front Plateau. Of the 4 cases listed, 2 showed significant improvement in initial signs and symptoms. Front Plateau may be a favourable treatment option for patients with TMD, if the guidelines are followed. Clinical trials on this modality should seek to minimize possible biases and limitations associated with the design of this type of research.

• Klíčová slova
• Conservative treatment, Occlusal splints, Temporomandibular joint, Temporomandibular joint disc, Temporomandibular joint disorders,
• MeSH
• akrylové pryskyřice terapeutické užití   MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• nemoci temporomandibulárního kloubu * terapie   diagnóza   MeSH
• prospektivní studie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• přehledy MeSH
• Názvy látek
• akrylové pryskyřice MeSH

The reaction between two equivalents of the Schiff base ligands N,N'-bis(3-methoxysalicylidene)ethylenediamine (H2L1) or enantiopure N,N'-bis(3-methoxysalicylidene)cyclohexane-1,2-diamine (H2L2) with one equivalent of Ce(NO3)3·6H2O in the presence of a bulky counteranion leads to the formation of chiral metal-organic frameworks (MOFs) whose channels encapsulate the counteranion, leading to the formation of compounds with the structural formulas {[Ce(NO3)2(L1)2]X·H2O}n, where X = ClO4- (1), PF6- (2), or BF4- (3), and {[Ce(NO3)2(L2)2]X·CH3CN}n, where X = ClO4- (4), PF6- (5), or BF4- (6), as well as the isostructural reference compound {Nd(NO3)2(L1)2]BF4·CH3CN}n (3Nd). A combination of static and dynamic magnetic measurements demonstrates the good isolation of the CeIII centers and a field-induced slow relaxation of the magnetization. Correlations between the temperature and field-dependent magnetic relaxation data and ultralow-frequency Raman spectroscopy reveal the presence of a vibronic barrier driving magnetic relaxation. Theoretical calculations have been performed to elucidate the nonparticipation of the electronic excited states in the main relaxation processes.

• Publikační typ
• časopisecké články MeSH

Lipid-mediated delivery of active pharmaceutical ingredients (API) opened new possibilities in advanced therapies. By encapsulating an API into a lipid nanocarrier (LNC), one can safely deliver APIs not soluble in water, those with otherwise strong adverse effects, or very fragile ones such as nucleic acids. However, for the rational design of LNCs, a detailed understanding of the composition-structure-function relationships is missing. This review presents currently available computational methods for LNC investigation, screening, and design. The state-of-the-art physics-based approaches are described, with the focus on molecular dynamics simulations in all-atom and coarse-grained resolution. Their strengths and weaknesses are discussed, highlighting the aspects necessary for obtaining reliable results in the simulations. Furthermore, a machine learning, i.e., data-based learning, approach to the design of lipid-mediated API delivery is introduced. The data produced by the experimental and theoretical approaches provide valuable insights. Processing these data can help optimize the design of LNCs for better performance. In the final section of this Review, state-of-the-art of computer simulations of LNCs are reviewed, specifically addressing the compatibility of experimental and computational insights.

• Klíčová slova
• ionizable lipid, lipid nanocarrier, lipid nanoparticle, liposome, molecular simulation, vesicle,
• MeSH
• léčivé přípravky chemie   MeSH
• lidé MeSH
• lipidy * chemie   MeSH
• nanočástice chemie   MeSH
• nosiče léků chemie   MeSH
• počítačová simulace MeSH
• simulace molekulární dynamiky * MeSH
• strojové učení MeSH
• systémy cílené aplikace léků metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• léčivé přípravky MeSH
• lipidy * MeSH
• nosiče léků MeSH

Pleuroparenchymal fibroelastosis (PPFE) is a rare fibrotic lung disease with a poor prognosis. Some patients with PPFE have prominent lesions in one upper lobe. Due to the unilateral nature of lesions, similarities between some patients of PPFE and unilateral upper field pulmonary fibrosis (unilateral upper-PF) have been indicated. A 55-year-old man was referred to our hospital with PPFE, which had developed dyspnea 9 months previously. He had undergone resection of renal cancer 64 months prior to the onset of PPFE. He was administered the antifibrotic drug nintedanib, but passed away due to respiratory failure 43 months after the onset. What were impressive points in the clinical course of this PPFE patient was the similarity to unilateral upper-PF, including his medical history, and the poor prognosis. Although very rare, we do believe that the information on medical history and progression in this patient might provide suggestion into the treatment of future patients with a similar trajectory.

• Klíčová slova
• Pleuroparenchymal fibroelastosis, Postoperative, Prognosis, Weight loss,
• MeSH
• fatální výsledek MeSH
• lidé středního věku MeSH
• lidé MeSH
• plicní fibróza * diagnóza   patologie   MeSH
• počítačová rentgenová tomografie MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

Eccrine hidrocystomas are rare, benign cystic lesions that usually affect the scalp, cheeks, and eyelids. They are thought to originate from the sweat glands. These lesions can be single or many in nature and frequently worsen in the summer from increased perspiration. They are caused by dilated ducts of eccrine sweat glands. Clinically, they seem like small, transparent cystic lesions that are painless, and they usually affect middle-aged or older people. However, a histological study is necessary to make a final diagnosis. We present a case of a 35-year-old woman who had an epidermal inclusion cyst first identified as a single, painless cystic growth on her right lower eyelid.

• Klíčová slova
• Apocrine hidrocystoma, Eccrine hidrocystoma, Eyelid swelling,
• MeSH
• diferenciální diagnóza MeSH
• dospělí MeSH
• ekrinní žlázy patologie   MeSH
• epidermální cysta * diagnóza   patologie   MeSH
• hidrocystom * diagnóza   patologie   MeSH
• lidé MeSH
• nádory očního víčka diagnóza   patologie   MeSH
• nádory potních žláz * diagnóza   patologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• přehledy MeSH

Iliac crest is common site for harvesting bone grafts. Morphometry of iliac crest is of vital importance in orthopedic surgery. Measurements were done on male (n=85) and female (n=85) hip bones. Length of iliac crest, thickness of iliac crest and ilium were measured. Thickness was measured at pre-defined points on crest and ilium 2 cm apart starting from anterior superior iliac spine (ASIS). Ilium was measured at a depth of 2.5 cm from crest. Statistical analysis was done. Iliac crests were longer in male bones. Ventral iliac crest was thickest at 6 cm from ASIS in both sexes. While iliac crest bore minimum thickness at 12 cm and 10 cm from ASIS in male and female bones respectively, however at 2.5 cm below iliac crest surface ilium was thickest at 4 cm from ASIS and at ASIS in male and female bones respectively. In case of male bones, dorsal part of iliac crest was thickest at 2.15 ± 1.29 cm from posterior superior iliac spine (PSIS) while in females it was at 1.78 ± 1.31 cm from PSIS. In dorsal part of ilium, it was observed at 2.31 ± 1.47 cm and 1.9 ± 1.79 cm from PSIS for male and female bones respectively. This study provided detailed variable morphometry and significant sexual dimorphism observed in iliac crest and ilium. Thickest safe zones in both sexes are a useful guide for harvesting appropriate bone grafts.

• Klíčová slova
• Hip bone morphometry, Iliac crest thickness, Ilium, Orthopedic reconstructive surgery,
• MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• odběr tkání a orgánů metody   MeSH
• os ilium * anatomie a histologie   MeSH
• senioři MeSH
• transplantace kostí * metody   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Oxalates are simple, low-cost but crucial compounds in the technology of lanthanides, actinides, and transition metals. Apart from using oxalate as a versatile ligand in coordination chemistry, simple oxalate salts are still under a scientific focus, linked with ion batteries, optical and magnetic materials, and, most importantly, industrial-technological mining and separation loops. The typically low solubility of oxalate salts is advantageous from the viewpoint of a convenient and affordable synthesis requiring only green solvents. Even though basic lanthanide oxalates have been known for decades, their structural descriptions have remained fuzzy, especially concerning water content and heavy lanthanide analogues. Herein, we present a newly developed preparation technique for large oxalate monocrystals applied to the whole lanthanide series. All of the structures were reviewed, and some new structures were determined. All of these oxalates exhibit a honeycomb structure with closed cavities containing water molecules. These honeycomb coordination polymers form a layered structure bonded by hydrogen bonds. Surprisingly, most oxalates can be easily exfoliated/delaminated in EtOH, forming colloids of up to single-layered nanosheets. Such a feature has never been described for 2D lanthanide oxalates and demonstrates a new form of applicability for them, e.g., for the construction of thin films or inkjet-printed layers using an extremely facile and economical preparation route.

• Publikační typ
• časopisecké články MeSH

In the clinical evaluation of upper extremity embolism cases, the anamnesis should focus on identifying potential triggering risk factors. The physical examination may reveal sensory deficits, aiding in the determination of ischemia stages. Imaging diagnosis is crucial, with computed tomography (CT) angiography being the preferred examination due to its ability to provide detailed information about arterial anatomy across multiple planes and clear visualization of adjacent structures. Compared to magnetic resonance imaging angiography, CT angiography offers faster results with minimal distortion, despite the exposure to radiation and contrast use. Doppler ultrasonography is another valuable tool in suspected arterial thromboembolism cases, particularly in emergency settings. It offers advantages over CT angiography as it is non-invasive, cost-effective, and does not involve radiation or contrast administration. We present the case of a 68-year-old man who reported a nodule in the medial region of his right thumb for three months. Initially, he experienced significant local pain and limited movement, which gradually improved over time with the use of analgesic medication. Ultrasonography revealed thrombosis in the princeps pollicis artery, and the patient commenced treatment with acetylsalicylic acid. After four months, the patient reported a marked reduction in the nodule size along with pain improvement.

• Klíčová slova
• Doppler, Princeps pollicis artery, Thrombosis, Ultrasonography,
• MeSH
• lidé MeSH
• palec ruky * krevní zásobení   MeSH
• senioři MeSH
• trombóza * diagnóza   diagnostické zobrazování   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH