Isoleucine added to the cultivation medium of Streptomyces cinnamonensis C-100-5 induced a relative increase of the production of monensin B at the expense of monensin A. U-14C-Isoleucine was found not to be a specific monensin B precursor. The incorporation of 1-13C-2-methylbutyrate into monensins A and B showed the label to be evenly incorporated in both products at carbon atoms originating from C(1) of propionate. In regulatory mutants insensitive to 2-amino-3-chlorobutyrate isoleucine influenced the production of monensins only slightly but strains resistant to 2-aminobutyrate and norleucine decreased their total production by 2-12% in the presence of isoleucine which was associated with a decrease of monensin A content by 14-52%. The inhibitory effect of isoleucine on the biosynthesis of valine, a specific precursor of the butyrate unit of monensin A, is discussed.

• MeSH
• aminobutyráty farmakologie   MeSH
• butyráty metabolismus   MeSH
• furany biosyntéza   MeSH
• isoleucin metabolismus   farmakologie   MeSH
• monensin analogy a deriváty   biosyntéza   MeSH
• mutace MeSH
• norleucin farmakologie   MeSH
• Streptomyces genetika   metabolismus   MeSH
• valin biosyntéza   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 2-amino-3-chlorobutyric acid MeSH Prohlížeč
• 2-methylbutanoic acid MeSH Prohlížeč
• aminobutyráty MeSH
• butyráty MeSH
• furany MeSH
• isoleucin MeSH
• monensin B MeSH Prohlížeč
• monensin MeSH
• norleucin MeSH
• valin MeSH