The role of the physical state of ovarian membranes was studied in immature rats after PMSG- and hCG-induced pseudopregnancy. Parallel changes in LH/hCG receptors, progesterone secretion and rigidity of membrane lipids were observed during pseudopregnancy. Possible structure-functional properties of the LH/hCG receptor were analyzed by the thermal perturbation technique. Thermal stability of the receptor was higher 5 days after hCG ovulatory injection to rats compared to days 11 or 18 and to control rats. Pseudopregnancy modified the quenching of protein fluorescence. The Stern-Volmer constants for controls and for rats on days 5, 11 and 18 of pseudopregnancy were found to be 2.4 and 4.6, 5.1 and 4.4, respectively, indicating that accessibility of fluorophores for the quencher was increased.

• MeSH
• buněčná membrána fyziologie   MeSH
• choriogonadotropin farmakologie   MeSH
• fluorescenční polarizace MeSH
• gonadotropiny koňské farmakologie   MeSH
• krysa rodu Rattus MeSH
• ovarium fyziologie   MeSH
• potkani Wistar MeSH
• progesteron krev   MeSH
• pseudogravidita chemicky indukované   patofyziologie   MeSH
• receptory LH fyziologie   MeSH
• vazebná místa MeSH
• vztahy mezi strukturou a aktivitou MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• choriogonadotropin MeSH
• gonadotropiny koňské MeSH
• progesteron MeSH
• receptory LH MeSH